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EC number: 229-782-3 | CAS number: 6731-36-8
Day of Study
Number of juveniles
1 (Number of adults)
2 (Number of adults)
3 (Number of adults)
Number of Juveniles 1 [N]
Number of Juveniles 2 [N]
Number of Juveniles 3 [N]
Total mortality of parent groups [%]
– = no juveniles
* = immobile / aborted juveniles
√ = First Brood/Juveniles present
M = Mortality
E= Aborted eggs
Day of sampling
Control Tank I
Control Tank II
Control Tank III
Control Inlet I
Analytical Quality Criteria
80 - 120
83.3 – 118.7
Quality Control (QC) samples (%)
Repeatability (CV (%)) at lowest standard
Repeatability (CV (%)) at Highest standard
System stability (% of nominal)
Recovery stability samples (%)
A GLP study to appropriate guideline with justified modifications considering test substance properties. Certificate of analysis and chemical analysis were present. A modified test setup to avoid high energy dispersions above solubility limit was used. Technical limits restricted the quantification limit of the analytical method to 4 µg/L. This prevented measurement (for the vast majority of vest vessel samples) in the test replicates themselves. This was due to the 2 µg/L solubility limit of the test material. Analysis did however demonstrate continual presence of the test material at an average concentration of 33.9µg/L in the stock (WAF) solution that was continually pumped into the test system. Furthermore the secondary separation vessel (directly feeding the test vessels) maintained an average concentration of 12.8 µg/L throughout the test period. This is considered the best attempt possible at this time due to technical limitations of the analytical apparatus used. Due to further separation taking place after the secondary separation vessel and potential reaction with organic material in the test vessels detection in the test vessels themselves was not possible in the majority of samples taken. However during the priming of the test system and occasionally throughout the test the inlet and tank measurements the test material was detected above the LOQ. It is therefore fair to assume that considering the continual dosing and relitively high nominal loading of 15 mg/L, that the best possible exposure took place in the test vessels. The study is not without limitations however.
No effects on the reproduction of Daphnia Magna were observed in a chronic flow through study at the limit of solubility of the test substance in the
A flow through study with a nominal loading of 15 mg/L of the test substance was conducted using a slow stir method (WAF) and two stage separation of the stock solution to avoid the testing of dispersed material. The stock (or large WAF solution 30L) was continually pumped into the test system the WAF itself was refreshed 3 times per week to ensure continual presence of the test material. Analytical measurements demonstrated the presence of the test material in the WAF solution throughout the test as well as in the secondary separation vessel at concentrations above the limit of solubility. From the secondary separation vessels triplicate test vessels containing 10 test organisms were exposed to the test substance for 21 days over which primarily the effects on reproduction were assessed.
No siginificant effects on reproduction or body length were observed. Indicating that 1,1-Di(tert-butylperoxy)-3,3,5-trimethylcyclohexane causes no chronic effects to Daphnia magna at it's water solubility limit. No chronic classification is required for this substance based on this data.
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