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Additional ecotoxological information

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additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
18-2-2015 - 11-3-2015
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP Supporting study. Not valid as a stand alone study. Reliable with restrictions when used in conjunction with existing key studies and T15006 CAS sewage water simulation study. Conducted according to guideline as Effluent Limit study.
Reason / purpose:
reference to other study

Data source

Reference Type:
study report
Report Date:

Materials and methods

Test guideline
equivalent or similar to
other: OECD 211
Conducted as an Effluent Limit test
Principles of method if other than guideline:
Test was conducted as an effluent test. Test organisms were exposed to undiluted control and treated effluent the assessment of effects took place
according to the guideline.
GLP compliance:
Type of study / information:
In parallel to sewage treatment simulation testing the investigation of the chronic effects of the collected effluent to Daphnia Magna was conducted in the form of a Non GLP supporting study.

Test material

Test material form:
other: Treated sewage effluent (reactor treating 6731-36-8)
Details on test material:
The test material was effluent collected from an activated sludge reactor treating 10 mg/L of Di-Tert-Butyl 3,3,5 Trimethylcyclohexylidene Diperoxide
(CAS 6731-36-8). See study T15006 CAS. Control effluent was also collected from the control reactor only treating domestic waste water. Effluent
was taken after hydraulic retention time had been reached and daily for 3 weeks thereafter. The effluent was not checked for biodegradation / removalprior to use nor was the reactor considered to be operating optimally at this time. Hence the effluent is considered to be representative to a realistic
worst case effluent from a reactor with an non adapted microbial component. Effluent was stored in the refrigerator prior to use to minimize any
changes in composition.

Unfiltered control and test effluent from study T15006 CAS without modification

Results and discussion

Any other information on results incl. tables

No significant difference on reproduction was detected with both of the statistical tests used (Heteroscedastic t Test & Wilcoxon Two-Sample Test) . Adult survival was slightly better in the treated effluent than in the control.

Applicant's summary and conclusion

In conclusion data demonstrates that treated effluent should not be considered chronically toxic to Daphnia Magna and hence treated effluent forms a relatively low risk to the environment. Toxicity by possible degradation products in the water phase was not detected.
Executive summary:

Non GLP supporting study (based on relevant guideline) in which the effects of effluent (from a reactor treating 6731 -36 -8) were tested on D magna. Short memo summary style report. Reliable as supporting evidence only.