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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1986-04-22 to 1986-04-29
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Purity: > 99 %
Impurities: 4-chlorotoluene, 3,4-dichlorotoluene and further isomeres of 2,4-dichlorotoluene

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Hoechst AG, Kastengrund, Germany
- Number of animals: 3
- Average weight at study initiation: 2,6 - 3,0 kg
- Age: 3 - 5 month

ENVIRONMENTAL CONDITIONS
- Temperature: 20 +/- 3 °C
- Humidity: 50 +/- 20 %
- Photoperiod: 12 h dark, 12 h light
- Diet: "Altromin 2123" ad libitum an 15 g hay per day
- Water: water (ad libitum)
- Housing: one animal per cage

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of test animal
Amount / concentration applied:
0,1 mL of 2,4-Dichlorotoluene in on eye of each animal
Duration of treatment / exposure:
24
Observation period (in vivo):
1 h, 24 h, 48 h,72 h and 7 days after application
Number of animals or in vitro replicates:
3

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: average of observation period
Score:
0
Reversibility:
fully reversible
Irritation parameter:
iris score
Basis:
mean
Time point:
other: average of observation period
Score:
0
Reversibility:
fully reversible
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: average of observation period
Score:
0.9
Reversibility:
fully reversible
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: average of observation period
Score:
0
Reversibility:
fully reversible

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Due to the result 2,4-dichlorotoluene is classified as not irritating.
2,4-dichlorotoluene is is not subject to specific labelling under current EU regulations.
Executive summary:

In an eye irritation study 0.1 mL of undiluted 2,4-dichlorotoluene was instilled into the conjunctival sac of one eye of New Zealand White Rabbits (3 animals) for 24 hours. After 24 h eyes were rinsed with physiological saline. The untreated eye served as control.

Animals were observed for 7 days.

Observation of the eyes occurred 1 h, 24 h, 48 h, 72 h and 7 days after application of the test substance.

Eye irritation was scored according to OECD GL 405.

The findings resulted in irritation scores of 0 for cornea, iris and chemosis and in an irritation score of 0,9 for conjunctivae.

Due to the result of this study 2,4-dichlorotoluene is classified as not irritating.

2,4-dichlorotoluene is not subject to specific labelling under current EU regulations.