2,4-dichlorotoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1981-10-23 to 1981-11-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

The fish were placed to the aquaria 65 hours before the test.
2,4-dichlorotoluene is insoluble in water. Therefore the test substance was homogenizing with a disperser (Ultra-Turrax) with a small quantity of water. This mixture was then given to the test water and distributed evenly. The stated amounts of 2,4-dichlortoluene refer not to the dissolved but to the total amount of 2,4-dichlorotoluene in water.
Duration of the test was 96 h. 10 fishes were exposed to 4; 6,3; 7,1; 8,0; 9,0; 10,0 100 mg/L 2,4-dichlorotoluene. 30 fishes were in a control group without exposure.
Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 2, 24, 48, 72, and 96 hours to be within the acceptable limits.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzene, 2,4-dichloro-1-methyl-

IUPAC Name:

Benzene, 2,4-dichloro-1-methyl-

Constituent 2

Reference substance name:

2,4-dichlorotoluene

EC Number:

202-445-8

EC Name:

2,4-dichlorotoluene

Cas Number:

95-73-8

Molecular formula:

C7H6Cl2

IUPAC Name:

2,4-dichloro-1-methylbenzene

Details on test material:

2,4-dichlorotoluene, colourless, liquid.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

2,4-dichlorotoluene is insoluble in water. Therefore the test substance was homogenizing with a disperser (Ultra-Turrax) with a small quantity of water. This mixture was then given to the test water and distributed evenly.

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Leuciscus idus f. melanotus
Source: Paul Eggers, Hohenwestedt
Weight: 1,4 - 2,6 g
Length: 5,4 - 6,7 cm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

48 h and 96 h

Test conditions

Hardness:

192 mg/L NaHCO3
120 mg/L CaSO4 ·H2O
120 mg/L MgSO4

Test temperature:

9 - 11 °C

pH:

pH 8,1 - 8,4

Dissolved oxygen:

> 8 mg/L

Salinity:

8 mg KCl

Nominal and measured concentrations:

nominal:
0 mg/L, 4,0 mg/L, 6,3 mg/L, 7,1 mg/L, 8,0 mg/L, 9,0 mg/L, 10 mg/L, 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

9 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not dissolved, emulsion

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

9 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not dissolved, suspension

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Conclusions:

From this study, LC50 for 2,4-dichlorotoluene was determined to be 9 - 10 mg/L (after 48 h and after 96 h).

Executive summary:

Testing laboratory was Deptmartment Pharma Forschung, Toxikologie at Hoechst AG, Frankfurt/Main. A static test with nominal test substance concentrations was conducted.

2,4-dichlorotoluene is insoluble in water. Therefore the test substance was homogenizing with a disperser (Ultra-Turrax) with a small quantity of water. This mixture was then given to the test water and distributed evenly.

Duration of the test was 96 h. 10 test organisms were exposed to 0; 4; 6,3; 7,1; 8; 9; 10 and 100 mg/L of 2,4-dichlorotoluene. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 24, 48, 72, and 96 hours to be within the acceptable limits. No lethality occurred in the control group.

At a concentration up to 9 mg/L no behavioural abnormalities were observed.

The highest examined concentration without lethality is 9 mg/L (after 48 h and after 96 h).

The lowest examined concentration with 100 % lethality is 100 mg/L (after 48 h and 96 h).

As a result, the LC50 value is 9 – 10 mg/L (after 48 h and 96 h).