Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23/3 1992 to 10/4 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: similar to guideline study under GLP, but no analytical confirmation of test concentrations and limited information on soil used

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p-menthane
- Physical state: liquid
- Stability under test conditions: stable (no details)

No additional information available

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

open allclose all
Species:
Triticum aestivum
Plant group:
Monocotyledonae (monocots)
Species:
Trifolium ornithopodioides
Plant group:
Dicotyledonae (dicots)
Species:
Lepidum sativum
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
other: seedling emergence and growth test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
18 d
Post exposure observation period:
none

Test conditions

Test temperature:
day 25 ◦C; night 20 ◦C
pH:
6.8
Moisture:
15% water added, measured 21-22%
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): plastic pot height 9-10 cm; Ø 8 cm
- Amount of soil: 200 g ww
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 5

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil): sand (USDA)
- % sand: 95
- % silt: 5 (partcles < 20um)
- % clay: 0
- Organic carbon (%): 0.18%
- not sieved

GROWTH CONDITIONS
- Photoperiod: 10/14 (L/D)
- Light source: Hg lamp
- Light intensity: growth fase day 10000 Lux; emergence fase ca. 300 Lux
- Day/night temperatures: 25/20 ◦C
- Relative humidity (%): 100%
- Air exchange rate: not indicated
- Watering regime and schedules: not indicated
- Volume applied: 15%

EFFECT PARAMETERS MEASURED :
- Emergence, growth on day 18 by fresh shoot weight
- Phytotoxicity rating system: none

VEHICLE CONTROL PERFORMED: yes

No additional information provided
Nominal and measured concentrations:
nominal: 1, 10 and 100 mg/kg dw
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Species:
Triticum aestivum
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: in controls 17/20
Species:
Triticum aestivum
Duration:
18 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Trifolium ornithopodioides
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: in controls 20/20 seeds emerged
Species:
Trifolium ornithopodioides
Duration:
18 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Lepidum sativum
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks on result:
other: in controls 17/20
Species:
Lepidum sativum
Duration:
18 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth

Any other information on results incl. tables

see attached document

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance did not induce toxicity to emerging and growing seedlings of three plant species
Executive summary:

In order to assess effects of the test substance on emergence and growth of seedlings of Triticum aestivum, Trifolium ornithopodioides and Lepidum sativum, seeds were planted and allowed to grow for 18 days. No effects on emergence and growth were seen at any of the concentrations tested (1, 10, 100 mg/kg dw mixed in soil).