Diallyl phthalate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Provisionnal Procedure: extended toxicology test with Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of the first appearance of offspring; 21 d)

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnid
- Strain/clone: Daphnia magna
- Feeding during test
- Food type: Tetramin-Hauptfutter (fish feed) and activated sludge
- Frequency: in open test vessels: daily; in closed test vessels: the days on which the parent animals were transferred

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: dry algae of the Scenedesmus genus (9 g food/ 1000 ml tap water)
- Feeding frequency: daily

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

16°

Test temperature:

25°C

Nominal and measured concentrations:

Nominal: 0.025-25 mg/l range

Details on test conditions:

TEST SYSTEM
- Type: open 400-ml vessels containing 250 ml of test solution and covered with watch glasses..
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

VEHICLE CONTROL PERFORMED: not relevant

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

reproduction

Remarks:

+ parent mortality + appearance of the first offspring

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mortality

Duration:

24 h

Dose descriptor:

other: EC0

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

not specified

Basis for effect:

mortality

Results with reference substance (positive control):

21d-NOEC=0.05 mg/l

Validity criteria fulfilled:

yes

Conclusions:

The long-term toxicity of diallyl phthalate to Daphnia magna is characterised by a 21-d NOEC value of 3.2 mg/l for reproductive capacity, appearance of first brood and parental mortality.

Description of key information

The key information is based on a peer reviewed published study performed under the supervision of the German authorities. Acute toxicity was in agreement with data from a GLP study reported elsewhere in this file. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.2 mg/L

Additional information

The long-term toxicity of diallyl phthalate to Daphnia magna is characterised by a 21-d NOEC value of 3.2 mg/l for reproductive capacity, appearance of first brood and parental mortality.