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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: Provisionnal Procedure: extended toxicology test with Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of the first appearance of offspring; 21 d)
Deviations:
no
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnid
- Strain/clone: Daphnia magna
- Feeding during test
- Food type: Tetramin-Hauptfutter (fish feed) and activated sludge
- Frequency: in open test vessels: daily; in closed test vessels: the days on which the parent animals were transferred

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: dry algae of the Scenedesmus genus (9 g food/ 1000 ml tap water)
- Feeding frequency: daily

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
16°
Test temperature:
25°C
Nominal and measured concentrations:
Nominal: 0.025-25 mg/l range
Details on test conditions:
TEST SYSTEM
- Type: open 400-ml vessels containing 250 ml of test solution and covered with watch glasses..
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

VEHICLE CONTROL PERFORMED: not relevant
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
reproduction
Remarks:
+ parent mortality + appearance of the first offspring
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
26 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
other: EC0
Effect conc.:
11 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mortality
Results with reference substance (positive control):
21d-NOEC=0.05 mg/l
Validity criteria fulfilled:
yes
Conclusions:
The long-term toxicity of diallyl phthalate to Daphnia magna is characterised by a 21-d NOEC value of 3.2 mg/l for reproductive capacity, appearance of first brood and parental mortality.

Description of key information

The key information is based on a peer reviewed published study performed under the supervision of the German authorities. Acute toxicity was in agreement with data from a GLP study reported elsewhere in this file.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
3.2 mg/L

Additional information

The long-term toxicity of diallyl phthalate to Daphnia magna is characterised by a 21-d NOEC value of 3.2 mg/l for reproductive capacity, appearance of first brood and parental mortality.