1-phenylethyl acetate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31st May 2011 to 10th June 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: A GLP compliant study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

- Name of test material (as cited in study report): 1-phenylethyl acetate
- Physical state: colourless to pale yellow liquid
- Name of test material (as cited in study report): Gardenol
- Lot/batch No.: VE00123242
- Expiration date of the lot/batch: 14th January 2013

Radiolabelling:

no

Details on study design: HPLC method:

EQUIPMENT- Apparatus: Perkin Elmer (series 200) HPLC system comprising: a high pressure pump (module M2910102), an auto-sampler (mode N2930100), a column compartment (module N2601581) and a refractive index detector (series 200a)- Type, material and dimension of analytical (guard) column: 250 mm x 4.6mmm Nucleodur® 100-5 CN-RP (Macherey-Nagel)- Flow rate: 1.0 mL/minute- Column temperature: 35ºC- Injection volume: 100 µLMOBILE PHASES- Type: methanol/water 55/45 (v/v)REFERENCE SUBSTANCES- Identity: 4 g/L formamide (CAS 75-12-7), 0.20 g/L 2-nitrobenzamide (CAS 610-15-1), 0.20 g/L methyl benzoate (CAS 95-58-3), 0.05 g/L naphthalene (CAS 91-20-3), 0.20 g/L 1,2,3-trichlorobenzene (CAS 87-61-6), 0.10 g/L phenanthrene (CAS 85-01-8), 0.20 g/L 4,4'-DDT (CAS 50-29-3). On the day of determination, a calibration mixture of these seven substances was prepared by mixing 2 mL of each of the individual solutions with enough ultrapure water to obtain a solvent composition similar to the mobile-phase (11.45 mL).PROCEDUREA solution of the test substance was prepared (12.5 mg in 50 mL methanol/water (55/45 v/v). After equilibration of the HPLC system, the calibration mixture was injected first followed by the test solution twice and the calibration mixture again. Retention times, tR, were measured and averaged and the decimal logarithms of the capacity factors, k, were calculated. The linear regression parameters of the relationshiplog koc = f(log k)were also calculated from the data obtained with the calibration mixture and, therewith, log Koc of the test substance was determined from its measured capacity factor.Finally, solutions of the reference substances were injected individually to confirm the peak assignment.

Type:

log Koc

Value:

2.41

Details on results (HPLC method):

- Retention times of reference substances used for calibration:Formamide: 4.682-nitrobenzamide: 5.29Methyl benzoate: 6.96Naphthalene: 9.981,2,3-trichlorobenzene: 11.06Phenathrene: 15.354,4'-DDT: 31.63- Details of fitted regression line (log k' vs. log Koc): Log Koc = a * log k + bCorrelation coefficient r = 0.9442, a = 2.5726, b = 3.0984- Graph of regression line attached: Yes- Average retention data for test substance: 7.21

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the test, the adsorption coefficient (log Koc) of the test substance was determined to be 2.41.

Executive summary:

In a GLP compliant adsorption-desorption coefficient study conducted in line with standardised guidelines OECD 121 and EU Method C.19, the adsorption coefficient (K_oc) of the test substance on soil and sewage sludge was determined by the Reverse Phase High Performance Liquid Chromatographic Method. Under the conditions of the test, the adsorption coefficient was determined to be log K_oc 2.41.

Description of key information

The adsorption coefficient of 1-phenylethyl acetate was determined to be log Koc 2.41 according to a study performed in line with OECD Guideline 121 and EU Method C.19

Key value for chemical safety assessment

Koc at 20 °C:

257.03

Additional information

The adsorption coefficient (K_oc) of 1-phenylethyl acetate on soil and sewage sludge was determined by the Reverse Phase High Performance Liquid Chromatographic Method and under the conditions of the test, the log K_oc was determined to be 2.41.

[LogKoc: 2.41]