Trisodium 7-(4-(4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazine- 2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Remarks:

IC50

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 February 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Sludge from a Husman laboratory apparatus

Qualifier:

equivalent or similar to guideline

Guideline:

ETAD Fermentation Tube Method

GLP compliance:

yes

Specific details on test material used for the study:

Batch No.: Op. 1/86., Vers. 10/86

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

no data

Test organisms (species):

other: mixed culture of bacteria (sludge of a Husman apparatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

Nominal and measured concentrations:

nominal concentrations: 0, 1, 3.2, 10, 32 and 100 mg/L

Details on test conditions:

O2 consumption, measured with an Oxygen-sensitive electrode system: OXI DIGI 550.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

No inhibition was observed except at 10 mg/L. There is no dose-response curve and there are no indications that the effects observed can be related to exposure to the testsubstance

Results with reference substance (positive control):

The IC50 of the reference substance 3,5-Dichlorophenol is: 21 mg/L (criterium: 10 - 25 mg/L)

IC50:

Calculate the respiration rate of each test concentration as a percentage of the mean of the two control respiration rates. Plot the results on a log (concentration)/normal (% inhibition) graph and derive an IC50 value.

	3 h
Test Substance (mg/1)	O2 -Consumption/(mg/l/min)	Inhibition %
100	1.82	2.1
32	2.13	0.0
10	1.66	10.7
3.2	2.89	0.0
1	1.87	0.0

 

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of FAT 40224/C is >100 mg/L.

Executive summary:

A bacteria growth inhibition test was performed under GLP following the OECD 209 guideline. The test concentration used in the study were 0, 1, 3.2, 10, 32 and 100 mg/L. No inhibition was observed except at 10 mg/L. There is no dose-response curve and there are no indications that the effects observed can be related to exposure to the test substance. No inhibition was observed except for a single inhibition point. However, this could not be related to the substance. Therefore, the IC50 was determined to be >100 m/L while the IC50 of the reference substance 3,5-Dichlorophenol is 21 mg/L (criterium: 10 - 25 mg/L).

Description of key information

The IC50 of the test substance was determined in a respiration inhibition test performed according to OECD guideline 209. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 0, 1, 3.2, 10, 32 and 100 mg/L test substance for 3 hours. The test concentration used in the study were 0, 1, 3.2, 10, 32 and 100 mg/L. No effects on algal respiration were observed. The IC50 was determined >100 mg/L. There is no dose-response curve and there are no indications that the effects observed can be related to exposure to the test substance. No inhibition was observed except for a single inhibition point. However, this could not be related to the substance. Therefore, the IC50 was determined to be >100 m/L while the IC50 of the reference substance 3,5-Dichlorophenol is 21 mg/L (criterium: 10 - 25 mg/L).

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L