Methyl 3-mercaptopropionate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study conducted according to OECD Guideline 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Principles of method if other than guideline:

N/A

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-mercaptopropionate

EC Number:

220-912-4

EC Name:

Methyl 3-mercaptopropionate

Cas Number:

2935-90-2

Molecular formula:

C4H8O2S

IUPAC Name:

methyl 3-sulfanylpropanoate

Details on test material:

- Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- Substance type: active
- Physical state: liquid
- Analytical purity: 99.75%

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
N/A

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: secondary effluent from a biologic treatment plant

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): : 2.6*E5 bacteria/mL medium
secondary effluent from a biologic treatment plant of Versailles, France.
- Laboratory culture: N/A
- Method of cultivation: N/A
- Storage conditions: N/A
- Storage length: N/A
- Preparation of inoculum for exposure: N/A
- Pretreatment: N/A
- Concentration of sludge: N/A
- Initial cell/biomass concentration: 2.6*E5 bacteria/mL
- Water filtered: N/A
- Type and size of filter used, if any: N/A

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

37.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: N/A

- Additional substrate: N/A
- Solubilising agent (type and concentration if used): N/A
- Test temperature: 22 deg. C
- pH: N/A
- pH adjusted: yes/no N/A
- CEC (meq/100 g): N/A
- Aeration of dilution water: N/A
- Suspended solids concentration: N/A
- Continuous darkness: yes/no: N/A
- Other: N/A


TEST SYSTEM
- Culturing apparatus: N/A
- Number of culture flasks/concentration: N/A
- Method used to create aerobic conditions: N/A
- Method used to create anaerobic conditions: N/A
- Measuring equipment: N/A
- Test performed in closed vessels due to significant volatility of test substance: N/A
- Test performed in open system: N/A
- Details of trap for CO2 and volatile organics if used: N/A
- Other: N/A


SAMPLING
- Sampling frequency: 0, 1, 5, 8, 14, 20, 27, 28 days
- Sampling method: N/A
- Sterility check if applicable: N/A
- Sample storage before analysis: N/A
- Other: N/A


CONTROL AND BLANK SYSTEM
- Inoculum blank: N/A
- Abiotic sterile control: N/A
- Toxicity control: N/A
- Other: Appropriate controls and blank system were used. Details were not available.


STATISTICAL METHODS: N/A

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

N/A

Test performance:

- 46% after 28 days
- 21% degradation in the 10 days window
- No inhibition of inoculum observed.

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

46

Sampling time:

28 d

Details on results:

N/A

BOD5 / COD results

Results with reference substance:

Please see Tables 1-3 below.

Any other information on results incl. tables

Table 1. CO2 produced (mg/day)

Test Day	0	1	5	8	14	20	27	28
Blank 1	0	2.1	3.1	2.5	3.2	3.2	2.9	0.8
Blank 2	0	2.3	9.5	2.4	3.0	1.9	2.2	0.4
Mean
Blank	0	2.2	6.3	2.5	3.1	2.1	2.5	0.6
Test 1	0	2.1	3.1	6.8	6.9	5.3	13.1	0.6
Test 2	0	1.8	4.4	0.6	6.4	5.4	18.4	0.9
Mean
Test	0	1.9	3.7	3.7	6.7	5.3	15.7	0.8
Ref substance	0	33.2	35.3	2.6	2.2	1.0	0.2	0.2
Inhibition	0	14.5	16.1	13.5	11.4	7.9	0.2	-0.1
Sterile	0	2.2	1.3	0.4	0.1	-1.4	0.3	0.2

Reference substance is Sodium Benzoate.

Table 2. Cumulative CO2 produced (mg)

Test Day	0	1	5	8	14	20	27	28
Blank 1	0	2.1	5.2	7.7	10.9	13.2	16.1	16.8
Blank 2	0	2.3	11.8	14.2	17.2	19.0	21.2	21.7
Mean
Blank	0	2.2	8.5	10.9	14.0	16.1	18.6	19.3
Test 1	0	2.1	5.2	11.9	18.9	24.1	37.2	37.8
Test 2	0	1.8	6.2	6.8	13.1	18.5	36.9	37.8
Mean
Test	0	1.9	5.7	9.3	16.0	23.1	37.1	37.8
Ref substance	0	33.2	68.5	71.1	73.3	74.3	74.5	74.7
Inhibition	0	14.5	30.6	44.1	55.5	63.4	63.6	63.6
Sterile	0	2.2	3.5	4.0	4.1	2.6	3.0	3.2

Reference substance is Sodium Benzoate.

Table 3. Degradation (%)

Test Day	0	1	5	8	14	20	27	28
Test 1	0	3	6	14	23	29	45	46
Test 2	0	2	7	8	16	22	45	46
Mean
Test	0	2	7	11	19	26	45	46
Ref substance	0	40	83	86	89	90	90	91
Inhibition	0	9	19	27	34	38	39	39
Sterile	0	3	4	5	5	3	4	4

Reference substance is Sodium Benzoate.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

46% degradation in 28 days; not readily biodegradable.

Executive summary:

N/A