Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-878-2 | CAS number: 100-68-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The relative biodegradation values calculated from the measurements performed during the test revealed no significant biodegradation of Thioanisole. In the toxicity control, Thioanisole was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, Thioanisole is designated as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Determination of ‘ready’ biodegradability: carbon dioxide (CO2) evolution test (modified Sturm test) with Thioanisole.
The study procedures described in this report were based on the OECD guideline No. 301B, 1992. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C. 4-C and the ISO International Standard 9439, 1999.
Thioanisole was a colourless to slightly yellowish liquid with a purity of at least 99 %. The test substance was tested in duplicate at approximately 17.5 mg/L, corresponding to 12 mg TOC/L. The organic carbon content was based on the molecular formula. The theoretical CO2 production (ThCO2) of Thioanisole was calculated to be 2.48 mg CO2/mg. Preparation was as much as possible performed under yellow light and/or dimmed light conditions.
The study consisted of six bottles:
-2 inoculum blanks (no test substance)
- 2 test bottles (Thioanisole)
- 1 positive control (sodium acetate) and
- 1 toxicity control (Thioanisole plus sodium acetate).
Since Thioanisole was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2 litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing he test substance. After vigorous mixing (vortex) the resulting suspension was added quantitativel to the test medium. The test solutions were continuously stired during the test, to ensure optimal contact between the test substance and the test organisms. Test duration was 28 days (last CO2-measurement on the 29thday).
The relative biodegradation values calculated from the measurements performed during the test revealed no significant biodegradation of Thioanisole. In the toxicity control, Thioanisole was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, Thioanisole is designated as not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
