Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only secondary literature (tables), based on UBA test programme
Qualifier:
equivalent or similar to guideline
Guideline:
other: DIN 38412, part 8
Principles of method if other than guideline:
Growth inhibition test with the bacterium Pseudomonas putida / Test conducted by WaBoLu; no report available.
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
in stock solution only
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated stock solution (water-accommodated fraction) was prepared by mechanically stirring of excess DIPN per 1 L medium for 48 h followed by separation of the insoluble material from the water phase using pre-conditioned glass fiber filter. The stock solution was diluted with dilution water to the desired concentration.
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Total exposure duration:
16 h
Test temperature:
25 °C
Nominal and measured concentrations:
no data
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Aeration: no

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, part 8

OTHER TEST CONDITIONS
- Adjustment of pH: no


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth inhibition


Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
> 0.16 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Conclusions:
No growth inhibition within the range of water solubility.
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract / only short communication, based on UBA test programme
Principles of method if other than guideline:
Method: Bioluminescence inhibition assay / test conducted by WaBoLu, BGA/Berlin; no report available.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Vibrio fisheri
Details on inoculum:
no data
Test type:
static
Water media type:
saltwater
Total exposure duration:
15 min
Details on test conditions:
TEST SYSTEM

no data
Reference substance (positive control):
not specified
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
> 0.1 mg/L
Validity criteria fulfilled:
not specified
Conclusions:
No inhibitory effect within the range of water solubility.
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
Method: Comparative study programme on the applicability and validation of this test system
GLP compliance:
not specified
Analytical monitoring:
no
Details on test solutions:
DIPN was solubilized and finely dispersed in DMSO/medium.
Test organisms (species):
Tetrahymena pyriformis
Details on inoculum:
no data
Test type:
static
Water media type:
freshwater
Total exposure duration:
24 h
Hardness:
no data
Nominal and measured concentrations:
no data
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
> 0.15 mg/L

No EC50 could be derived, no data available on effects induced
by DIPN. 

It can be concluded that DIPN produced no toxicity within its water solubility. 
Documentation in tabular form only.
   

Validity criteria fulfilled:
not specified
Conclusions:
No inhibitory effect within the range of solubility

Description of key information

Diisopropylnaphthalene does not exhibit inhibitory effects on the microorganism tested within its range of water solubility (0.1 to 0.2 mg/L).

Key value for chemical safety assessment

EC50 for microorganisms:
0.15 mg/L

Additional information

For assessment of the toxicity to microorganisms, three studies have been identified. Due to limited reporting (secondary literature or short communication in tabular form), the reliability is 4. As results are very similar, they are used in a weight of evidence approach.

 

Study

Test concentration

Result

Experimental conditions

Reliability

UBA/KBwS 1999
(Pseudomonas putida, growth inhibition)

no individual test concentrations reported

EC10: > 0.16 mg/L
(no growth inhibition within the range of water solubility)

no vehicle
16 h
static

4 (WoE)
(secondary literature)

UBA/KBwS 1999
(Vibrio fischeri, luminescence inhibition)

no individual test concentrations reported

EC50: > 0.1 mg/L
(no inhibitory effect within the range of water solubility)

no vehicle
15 min
static
saltwater

4 (WoE)
(secondary literature)

Yoshioka 1985
(Tetrahymena pyriformis,

no individual test concentrations reported

EC0: > 0.15 mg/L
(no toxicity within the range of water solubility)

Vehicle: DMSO
24 h
static

4 (WoE)
(short communication)

 

Based on data reported, it is concluded that diisopropylnaphthalene does not exhibit inhibitory effects on the organism tested within its range of water solubility (0.1 to 0.2 mg/L).