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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
Oleaginous yeast, Lipomyces starkeyi (IFO10381) was maintained on YM agar. The compound as a methanol solution was added to 5 mL of broth in a test tube to produce final concentrations of 20 or 200 mg/L compound and 1% (v/v) methanol at the start of the culture. As a control, 1% methanol was added to the broth. Yeasts were cultivated using a rotary shaker (120 rpm) at 27 °C for 7 days
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
The compound was used as a methanol solution to produce final concentrations of 20 or 200 mg/L compound and 1% (v/v) methanol.
Test organisms (species):
other: Lipomyces starkeyi IFO10381
Details on inoculum:
Oleaginous yeast, Lipomyces starkeyi IFO10381 (IFO, Osaka, Japan) was maintained on YM agar at 25 °C. One loopful of yeast well grown on a YM agar slant was inoculated into 5 mL of YM broth and cultivated with 120 rpm rotary shaking at 27 °C for 2 days. The precultured broth was inoculated with 1% (v/v) inoculum into the MLA broth
Test type:
semi-static
Water media type:
not specified
Total exposure duration:
7 d
Test temperature:
27 °C
Nominal and measured concentrations:
20 and 200 mg/L
Details on test conditions:
The compound as a methanol solution was added to 5 mL of broth in a test tube to produce final concentrations of 20 or 200 mg/L compound and 1% (v/v) methanol at the start of the culture. As a control, 1% methanol was added to the broth. Yeasts were cultivated using a rotary shaker (120 rpm) at 27 °C for 7 days
Duration:
7 d
Dose descriptor:
other: EC14
Effect conc.:
20 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
growth inhibition
Remarks:
inhibited fungal growth by 14%
Duration:
7 d
Dose descriptor:
other: EC14
Effect conc.:
200 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
growth inhibition
Remarks:
inhibited fungal growth by 14%
Conclusions:
Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.
Executive summary:

Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.

Description of key information

Toxicity to microorganisms:

Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.

Key value for chemical safety assessment

EC50 for microorganisms:
200 mg/L

Additional information

Toxicity to microorganisms:

Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.