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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Test substance preparation and exposure 2010-04-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study performed according OECD 203 and GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: 25.09%
- Production date: 04/11/2009
- Lot/batch No.: 200911040014
- Shelf life: 6 months
- Date of sample receipt: 03/12/2009

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentration: 100 mg/l
- Sampling method:Representative water samples of exposure medium, control and solvent control was collected during the conduct of the experiment at 0 and 24h for analytical analysis.
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg of test item (for 10L) was weighed and directly transferred to the exposure medium.
- Dilution water (exposure medium): well water and soft water (reverse osmosis water) in the mixture of 1:1 ratio.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Brachydanio Rerio
- Strain: no data
- Source: supplied from a commercial fish farm.
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2 +- 1 cm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: /
- Feeding during test
- Food type: commercially available aquarium fish feed
- Frequency: daily


ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercially available aquarium fish feed
- Feeding frequency: feeding was stopped 24h prior to the commencement of the experiment
- Health during acclimation (any mortality observed): healthy


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
Observations for mortality and abnormal behaviour were made twice on the first day (approximately 3h and 6h after treatment) of the exposure, thereafter at the end of every 24 h until 96 h, when the experiment was terminated.

Test conditions

Hardness:
220 mg/L CaCO3
Test temperature:
control: 23.8-24.7 °C
100 mg/L: 23.9-24.7 °C
pH:
control: 7.68-7.94
100 mg/L: 7.67-8.01
Dissolved oxygen:
control: 7.58-8.18 mg/L
100 mg/L: 7.88-8.43 mg/L
Salinity:
NO DATA
Nominal and measured concentrations:
nominal 100 mg/L
average measured concentration is 90.16 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:glass aquaria (10l)
- Material, size, headspace, fill volume: 10l
- Renewal rate of test solution (frequency/flow rate):renewed daily
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):5
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water and soft water (reverse osmosis water) in the mixture of 1:1 ratio
- Total organic carbon: less than 5 mg/L
- Particulate matter:
- Metals: most of metals were below detection limit (exept B 0.0218 mg/L)
- Pesticides: PCBs measured were below detection limit
- Ca/mg ratio:
- Conductivity:618-636 µS/cm



OTHER TEST CONDITIONS
- Photoperiod:12h light, 12h darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 90.16 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: no signs of toxicity or behavioural abnormalities were observed in fish exposed to control and 100 mg/L.
- Mortality of control: no mortality was observed in control group and fish exposed to 100 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96h-LC50 of Sodium vinyl sulphonate solution (Provichem 2202) was greater than 100 mg/L.