Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study according GLP and OECD 121.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report):Sodium vinyl sulphonate (SVS) solution (Proviplast 2202)
- Analytical purity: 25.09%
- Lot/batch No.: 200911040014
- Storage condition of test material: ambient room temperature 20 to 30°C
- Other:
Radiolabelling:
not specified

Study design

Test temperature:
Column compartment temperature was maintained at 40°C.

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC-Agilent 1200 series
- Type: a reverse-phase, isocratic HPLC at 1.0 mL/minute flow rate and a 1.0 microL injection volume was used
-columln: a cyanopropyl column of length 250 mm x 4.6 mm id x 5 microm particle size, supplied by M/s. E.Merck India Ltd. was used
- Detection system: DAD detector at 220 nm, bandwidth 4 nm supported by chem station software

MOBILE PHASES
- Type: methanol/water 30:70 (v/v)
- Solutes for dissolving test and reference substances: methanol

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column which was formamide

REFERENCE SUBSTANCES
- Identity: formamide, acetanilide, aniline, atrazine, methyl benzoate, N,N-dimethyl benzamide, phenol

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column:1.0 microL injection volume was used

REPETITIONS
- Number of determinations: standerds and samples were analyzed in duplicate

EVALUATION
- Calculation of capacity factors k':log k': -0.32; -0.35; 0.32; 0.05; 0.05; -0.43 for acetanilide, phenol, anilide, atrazine, methyl benzoate, N,N-dimethyl benzamide, respectively
- Calculation of retention times: measured retantion times of reference substances: 4.365; 2.295; 9.162; 6.254; 6.292; 4.067 min for acetanilide, phenol, anilide, atrazine, methyl benzoate, N,N-dimethyl benzamide, respectively
- Determination of the log Koc value: 1.25; 1.32; 2.07; 1.81; 1.80; 1.52 for acetanilide, phenol, anilide, atrazine, methyl benzoate, N,N-dimethyl benzamide, respectively

Batch equilibrium or other method

Analytical monitoring:
not specified
Details on sampling:
no data

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
25.59
Type:
log Koc
Value:
1.41

Applicant's summary and conclusion

Conclusions:
The Koc value for SVS was determined to be 25.59 +/-0.013 (Log Koc was 1.41)