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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Good study according to GLP and OECD guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report):Sodium vinylsulphonate
- Physical state: Pale yellow liquid
- Analytical purity: 25.4%
- Impurities (identity and concentrations): 74.6% water and inert ingredients
- Lot/batch No.: 2B20AA
- Expiration date of the lot/batch: 27 may 1993
- Storage condition of test material: at +4°C

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in test water to give an stock solution of 400 mg/l (equivalent to 101.6 mg active ingredient/l)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Straus
- Source: Laboratory culture originating from a strain supplied by the institute national de recherche chimique appliquée (IRChA), France
- Age at study initiation (mean and range, SD): less then 24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
171 mg CaCO3/l
Test temperature:
21°C
pH:
8.0
Dissolved oxygen:
7.9 mgO2/l
Nominal and measured concentrations:
Nominal : 100 mg a.i./l
Measured : 94 mg a.i./l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass jars loosely covered with aluminium foil to limit evaporation losses
- Material, size, headspace, fill volume: 200 ml of prepared test medium or diluent water only
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water filtered, dechlorinated and softened by passage through an Elga water purification system. The water passes through a high pressure sand filter to remove colloids and particulate matter and a high grade activated carbon filter to remove chlorine and any organic contaminants. A proportion of the supply then passes through a water softener before final reverse osmosis treatment to produce a highly purified water supply. The two grades of dechlorinated water are then remixed in the ratio of approximately 1:1 to give a supply with the desired water hardness. Batches of softened water were "aged" with aeration for at least 12 hours prior to use by recirculating through a gravel filter bed. Chlorine levels remained below the limit of detection at all times and hardness was adjusted to 171 mg CaCO3/l.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility

Applicant's summary and conclusion