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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2022 November
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
Reaction mass of disodium 2,2 oxydiethanesulfonate and sodium ethenesulfonate
Batch lot number:202206140027
Dry weight content : 33.94 %, correction factor is 2.94 The test item is a multi-constituent
Composition.
Constituent1:Sodium ethylene sulphonate cas 3039-83-6: 74.84 %
Constituent 2 : Isethionate bisether, disodium salt cas 63440-92-6: 15.18%
Impurity 1:Isethionate sodium cas 1562-00-1: 5.15%
Impurity 2:Sodium ethionate, disodium salt cas1562-03-4: 0.48 %
Impurity 3:Sodium sulfate cas 7757-82-6 :3.12 %
Impurity 4:Sodium ethandisulfonate disodium salt cas 5325-43-9:0.49 %
Analytical monitoring:
yes
Details on sampling:
Samples for possible analysis were taken from the limit concentration and the control according to the schedule below. The method of analysis is described in the appended Analytical Report (Appendix 1).
Frequency 1. At the start of the test and after 24 hours from the freshly prepared solutions.
2. At the first renewal (t=24 h) and the end of the test from the 24-hour old solutions.
Volume 1.5 mL from the approximate center of the test solutions.
Storage Samples were stored in a freezer (set to maintain -20°C) until analysis at the analytical laboratory of the Test Facility.
At the end of each refreshment period, the replicates were pooled at each concentration before sampling.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia Magna
- Strain/clone: Crustacea cladocera straus
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD):Daphnia, less than 24 hours old, from parental daphnids of more than two weeks old.
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Stage and instar at study initiation:
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Source:
- Age of parental stock (mean and range, SD):
- Feeding during test; Daily, suspension of freshwater algae
- Food type: freshwater algae
- Amount:
- Frequency: daly

ACCLIMATION
- Acclimation period: no
- Acclimation conditions (same as test or not):
- Type and amount of food:no feeding
- Feeding frequency:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
Test type:
semi-static
Water media type:
freshwater
Remarks:
Culture medium M7 The following salts and vitamins were added to freshly prepared test medium (see section 4.9.2) to reach the following concentrations: Salts: H3BO3 0.71 mg/L FeSO4.7H2O 0.25 mg/L MnCl2.4H2O 0.090 mg/L LiCl 0.076 mg/L RbCl 0.018
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
Semi-static, with renewal of test solutions after 24 hours.
Glassware was wrapped in aluminum foil and preparation of test solutions was performed under dimmed light conditions to minimize exposure to light.
Post exposure observation period:
no
Hardness:
180 mg/L as CaCo3
Test temperature:
The temperature continuously measured in a temperature control vessel varied around 20°C during the test and complied with the requirements as laid down in the study plan (18 22°C, constant within ±1°C).
pH:
see table 3
Dissolved oxygen:
see table 3
Salinity:
no measured
Conductivity:
not measured
Nominal and measured concentrations:
see table 1,2,3,4, from the analytical report.Samples taken from the limit concentration and the control were analyzed for the two monitored constituents of the test material. The measured concentrations at the start of the test and after 24 hours from the freshly prepared solution were generally around nominal at the limit concentration for both constituents. The 24-hour old solutions showed that the concentrations remained stable over time.
Based on these results, effect parameters were expressed as analytically confirmed nominal concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel:60 ml glass vessels
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution:60 ml
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):renewal after 24 hours
- No. of organisms per vessel: 20 per concentration
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):no
- Biomass loading rate: 5 per vessel containing 50 ml of solution.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Yes after 24 h

VEHICLE CONTROL PERFORMED: yes, the test item is dissolved in water, the control is with water

RANGE-FINDING STUDY
- Test concentrations: (0,1.0,10 and 100 mg/L) for 48 h.
- Results used to determine the conditions for the definitive study:No immobility was observed in the control and at any of the test concentrations throughout the test. Therefore, the expected EC50 was above a nominal concentration of 100 mg/L. ( see Appendix2)
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other:
Remarks on result:
other: no toxicity was observed
Results with reference substance (positive control):
Test Facility Study No. 20415602
Experiment performed Nov 2022.
The batch of Daphnia magna tested, showed expected sensitivity to Potassium dichromate (48h-EC50: 0.64 mg/L with a 95% confidence interval ranging from 0.56 to 0.76 mg/L) based on the historical range of reference tests performed by the Test Facility in the last five years.
The raw data from this study are kept in the Charles River Den Bosch archives. The test described above was performed non-GLP.
Validity criteria fulfilled:
yes
Remarks:
1. In the control, no daphnids became immobilized or showed other signs of disease or stress, for example discoloration or unusual behavior such as trapping at the surface of the medium. 2. The dissolved oxygen concentration at the end of the test was ≥3
Conclusions:
In conclusion, the 48h-EC50 for Daphnia magna exposed to Reaction mass of disodium 2,2’ oxydiethanesulfonate and sodium ethenesulfonate ( EC: 700-978-5) was beyond the limit concentration tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.No immobility was observed in the control and at the limit concentration throughout the exposure period.(table 1)

Executive summary:

The objective of the study was to evaluate Reaction mass of disodium 2,2’‑oxydiethanesulfonate and sodium ethenesulfonate for its ability to generate acute toxic effects on the mobility of Daphnia magna during an exposure period of 48 hours and, if possible, to determine the EC50 at 24 and 48 hours of exposure.


A correction was made for the water content of the test material. A correction factor of 2.946 was used. All concentrations reported are based on dry residue.


A limit test was performed based on the results of a non-GLP preliminary range-finding test performed under a separate project, namely Test Facility Refence No. 20332242. The study met the acceptability criteria prescribed by the study plan and was considered valid. Test conditions and results of the limit test are presented in the table below:






























Test Material



Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate ( EC 700-978-5)



Appearance



Clear light-yellow liquid



Dry residue



33.94%



Correction factor



2.946



Preparation of test solutions



Nominal concentrations (mg/L)



Specific procedures



Glassware was wrapped in aluminum foil and preparation of test solutions was performed under dimmed light conditions to minimize exposure to light.



The validation criteria  are fulfilled , for this study.  Also the same batch of DM


 

Description of key information

In conclusion, the 48h-EC50 for Daphnia magna exposed to Reaction mass of disodium 2,2’ oxydiethanesulfonate and sodium ethenesulfonate ( EC: 700-978-5) was beyond the limit concentration tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.No immobility was observed in the control and at the limit concentration throughout the exposure period.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
> 100 mg/L

Additional information