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EC number: 281-339-3 | CAS number: 83929-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study (OECD TG 429), with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Methyl 4-[[(2,5-dichlorophenyl)amino]carbonyl]-2-nitrobenzoate
- EC Number:
- 281-339-3
- EC Name:
- Methyl 4-[[(2,5-dichlorophenyl)amino]carbonyl]-2-nitrobenzoate
- Cas Number:
- 83929-47-9
- Molecular formula:
- C15H10Cl2N2O5
- IUPAC Name:
- methyl 4-[(2,5-dichlorophenyl)carbamoyl]-2-nitrobenzoate
- Details on test material:
- - Name of test material (as cited in study report): Nitro-MMT-Dichloranilide
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- strain: CBA/CaOlaHsd
- Source: Harlan Netherlands
- Age at study initiation: 7-8 weeks
- Weight at study initiation: mean 18.8 g +/- 1.1 g (range 17.7-21.4 g)
- Housing: individually, Makkrolon Type I cages
- Diet: pelleted standard died (Harlan Winkelmann, Borchen), ad libitum
- Water: tap water, ad libitum
- Acclimation: yes (no information on duration)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 30-92
- Photoperiod (hrs dark / hrs light): 12 hrs/12 hrs
Study design: in vivo (LLNA)
- Vehicle:
- dimethyl sulphoxide
- Concentration:
- 0, 5, 10, 20% (w/v)
- No. of animals per dose:
- 4 females per dose group
2 females in the pre-test - Details on study design:
- RANGE FINDING TESTS:
- Compound solubility: 20% (w/v) suspension in DMSO was the highest technically applicable concentration; higher concentrations could also not be achieved with other vehicles
- Irritation: No irritation effects were observed after tripple application of test item concentrations of 2.5, 5. 10, and 20% (w/v).
- lymph node proliferation response: no data
MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: LLNA
- Criteria used to consider a positive response:
1: exposure to at least one concentration of the test item resulted in an incorporation of 3H-methyl thymidine at least 3-fold or greater than that recorded in control mice as indicated by the stimulation index
2: data are compatible with a conventional dose response, although allowance must be made for either local toxicity or immunological suppression especially at high topical concentrations
TREATMENT PREPARATION AND ADMINISTRATION:
- individual preparation of weight volume dilutions using a magnetic stirrer as homogenizer
- test item preparations were made freshly before each dosing occasion
- 25 µl were spread over the entire dorsal surface of each ear lobe once daily for three consecutive days - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- - calculations of mean values and standard deviations for body weight
Results and discussion
- Positive control results:
- Stimulation indices of 2.43, 4.07 and 4.88 were determined with the positve control substance at concentrations of 5%, 10% and 25% (w/v), respectively, in acetone:olive oil (4+1). An EC3 value of 6.7% (w/v) was calculated.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Stimulation indices were all below 3. The following SI were calcualted: 5% test item: 2.2 10. % test item: 1.5 20% test item: 0.8
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- There was no dose dependent increase in the dpm, which were measured for the pooled lymph nodes of each treatment group (8 lymph nodes per dose group): Background: 24 or 20 dpm Control group: 6360 dpm 5% test item: 13779 dpm 10% test item: 9638 dpm 20% test item: 4931 dpm
Any other information on results incl. tables
The animals did not show any clinical signs (neither local toxicity nor systemic findings) during the course of the study and no cases of mortality were observed. The body weights of the animals were within the range commonly recorded for animals of this strain. Calculation of the EC3 value was not performed, because no test concentration produced a SI of 3 or higher.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: other: CLP
- Conclusions:
- The test substance was not sensitising in this LLNA in concentrations up to 20% (w/v) in DMSO, the highest technically achievable concentration.
- Executive summary:
The test item was assessed for its possible contact allergenic potential in a local lymph node assay (OECD TG 429). For this purpose the test item was applied to the dorsal surface of the ears of female mice (4 per group) at concentrations of 5, 10, and 20% (w/v) in dimethyl sulphoxide for three consecutive days. The animals did not show any clinical signs during the course of the study and no cases of mortality were observed. In this study Stimulation Indices (S.I.) of 2.2, 1.5, and 0.8 were determined with the test item at concentrations of 5, 10, and 20%, respectively. The results obtained with the positive control confirmed the validity of the test. The test item was not a skin sensitiser in this assay.
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