Registration Dossier
Registration Dossier
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EC number: 618-126-5 | CAS number: 88196-70-7
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2007-11-29 to 2007-11-30 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 82796-69-8
- Cas Number:
- 82796-69-8
- IUPAC Name:
- 82796-69-8
- Details on test material:
- - Batch-Identification: 76584075L0
- Purity: 99.8 area-%
- Homogeneity: Homogeneous
- Storage stability: Expiry date: 17 January 2009
- Physical state/ Appearance: Liquid / colorless, clear
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2 g of the test substance were added to about 800 mL of demineralized water and stirred at room temperature until the test substance was completely dissolved. The stock solution was made up to 1 L with demineralised water. The stock solution appeared colorless and clear.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source and preparation of the inoculum: Activated sludge from the municipal wastewater treatment plant of the city Mannheim/Baden Württemberg (Germany). It was collected at 29 Nov 2007 from the aeration tank of the plant. The activated sludge suspension was washed thrice with tap water, sieved with a fine woven mesh (mesh size about 1 mm). This suspension was adjusted to a concentration of 7.5 g/L dry weight and pre-aerated over night. After the pre-aeration the lost water was replaced. This suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- Adjusted to 7.2
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L - Nominal and measured concentrations:
- Nominal concentrations: 62.4, 125, 250, 500 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of medium: According to guideline
- Intervals of water quality measurement: At test start and at test end
EFFECT PARAMETERS MEASURED: Respiration inhibition
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenole
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 230 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- 740 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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