Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 618-126-5 | CAS number: 88196-70-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2008-01-15 to 2008-01-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 82796-69-8
- Cas Number:
- 82796-69-8
- IUPAC Name:
- 82796-69-8
- Details on test material:
- - Batch-Identification: 76584075L0
- Purity: 99.8 area-%
- Homogeneity: Homogeneous
- Storage stability: Expiry date: 17 January 2009
- Physical state/ Appearance: Liquid / colorless, clear
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed. The samples were transported to the Analytical Laboratory on the day of sampling. The samples from end of exposure were brietly stored in the freezer, prior to transport due to a technical delay at the analytical laboratory. The retained samples were stored in the freezer.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution (100 mg/L) was prepared by dissolving of 200 mg of test substance in 2 L test medium and stirring for approximately 10 min at 20 ± 2 °C. The solution was neutralized with 1 M HCl to a pH-value equivalent to the control test media and then checked for complete dissolution of the test substance. The lower test concentrations were prepared by dilution of this neutralized solution. All test solutions were visibly clear over the exposure period.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE, Ludwigshafen Germany.
- Age at study initiation: < 24 h
- Feeding during test: No feeding during the exposure period.
CULTURE CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Water quality: See 3.3.1.
- Temperature: 20 ± 1 °C
- Diet: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding: Daily
- Renewal of the culture water: Twice weekly
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.20 – 3.20 mmol/L
- Test temperature:
- 20.2 – 21.0 °C
- pH:
- 7.9 – 8.1
- Dissolved oxygen:
- 8.4 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 10, 22, 50 and 100 mg/L
The analytically determined concentrations of the test substance in the test solutions were within ± 20% of the nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL, fill volume 10 mL)
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Acid capacity up to pH 4.3: 0.80 – 1.00 mmol/L
- Conductivity: 550 - 650 μS/cm
- Intervals of water quality measurement: At test start and at test end
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light: Artificial light, type warm white (OSRAM L58 W31), 390 – 480 Lux at a wave length of 400 - 750 nm
EFFECT PARAMETERS MEASURED: Mobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes, in a preliminary range finding test (experimental conduct in accordance with GLP but without a GLP status) the 48 hour EC50 was between 10 and 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 22.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Test concentrations were analytically verified.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Test concentrations were analytically verified.
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 98.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Test concentrations were analytically verified.
- Details on results:
- - Immobility of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: All of the test solutions were visibly clear over the exposure period.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: The EC50(24h) of the reference substance potassium dichromate was 1.01 mg/L (experiment date: 18 Dec 2007, project number: 50E0063/043039).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
