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EC number: 953-178-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 - 23 Sep 2011
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Council Regulation (EC) No 440/2008, Acute toxicity for Daphnia
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Source: CEFAS Laboratories, Lowestoft
- Date of gravid isolation: 20 Sep 2011
- Age of Daphnia at test start: Less than 24 h
- Culture conditions: Cultured under static conditions (20 °C, > 60% ASV, 16 h light and 8 h dark photoperiod)
- Feeding regime: A suspension of Chlorella vulgaris (as 1 mg organic carbon/L) every working day, until 24 h prior test start. The Daphnia were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 156 mg/L CaCO3
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.3 - 7.8
- Dissolved oxygen:
- 60% air saturation
- Nominal and measured concentrations:
- 0, 7, 13, 25, 50, and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 mL disposable plastic vessel, filled with 25 mL test medium and covered with a transparent perspex sheet
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The stocks of animals were cultured and the tests performed in reconstitued ISO water. The water was adjusted to 18 - 22 °C, aerated until the dissolved oxygen concentration had reached a minimum of 60% air saturation and the pH stabilised between 6 and 9.
- Hardness: 156 mg/L CaCO3
- Culture medium different from test medium: Culture medium same as test medium.
- Intervals of water quality measurement: Water quality, pH, and dissolved oxygen were measured in pooled replicates for each test and control solution immediately before the test and at the end of the 48 h test period. Temperature was recorded at 0,2 4, and 48 h.
OTHER TEST CONDITIONS
- Adjustment of pH: The pH stabilised between 6.0 and 9.0 during aeration of the test water.
- Photoperiod: 16 h light and 8 h dark
EFFECT PARAMETERS MEASURED:
- Immobilisation: After 24 h and 48 h
RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1, 10 and 100 mg/L (48 h)
- Results used to determine the conditions for the definitive study: Yes, 100% immobilisation at 100 mg/L after 48 h and no effects at lower concentrations. Data from the preliminary study identified the EC50 (48 h) as being between 10 and 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- None (0%) of the twenty control Daphnia maintained in dilution water were immobilised during the study, and no Daphnia were trapped at the surface of the water in the control vessels. Thus, the validity criteria of the study which states that control immobilisation must not exceed 10% at the end of the test was fulfilled.
The full water quality measurements are given in the summary tables (see any other infomation on results section) and were within acceptable limits. Specifically, the dissolved oxygen concentration remained greater than 3mg/l, which fulfils the validity criteria of the study. - Results with reference substance (positive control):
- Please see "Any other information on results section" for reference test results table.
- Reported statistics and error estimates:
- The ECx values with 95% confidence limits were calculated according to the methods of ToxCalcTM Version 5.0 "Comprehensive Toxicity Data Analysis and Database Software", copyright 1994-1996.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- The 48 hour EC50 value of Monoesters of C16 and C18 (branched and linear) fatty acids with decan-1-ol to Daphnia magna was estimated to be >100 mg/L.
- Executive summary:
A study was performed to investigate the acute toxicity of Monoesters of C16 and C18 (branched and linear) fatty acids with decan-1-ol to Daphnia magna. The objective of this study was to determine the 48 hour EC50 value, which is defined as the concentration that immobilises 50% of the exposed Daphnia after a 48 hour exposure period at 19 to 21 °C. The test was carried out according to the procedures based on the OECD 202 guideline Daphnia sp. Acute Immobilisation Test, Annex V EEC C2 Acute toxicity for Daphnia and ToxCalc Version 5.0 "Comprehensive Toxicity Data Analysis and Database Software". The 48 hour EC50 value of Monoesters of C16 and C18 (branched and linear) fatty acids with decan-1-ol to Daphnia magna was estimated to be >100 mg/L.
Reference
VALIDITY CRITERIA
The test met the validity criteria defined by the guideline and is thus considered valid and reliable (Table 1).
Table 1: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% immobilization was recorded in the control vessels. |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
The dissolved oxygen concentration was ≥ 3 mg/L. |
Yes |
WATER QUALITY DATA
Water quality data are summarized below (Tables 2 – 4). Water quality parameters were measured in pooled replicates for each test and control solution and were carried out immediately prior to initiating the test, at 24 h and at the end of the 48 h test period.
Table 2. Summary of pH values
Exposure period [h]
|
Concentration [mg/L] |
|||||
|
Control |
7 |
13 |
25 |
50 |
100 |
0 (fresh) |
7.8 |
7.7 |
7.6 |
7.6 |
7.5 |
7.5 |
48 (aged) |
7.3 |
7.4 |
7.4 |
7.4 |
7.4 |
7.5 |
Table 3. Summary of dissolved oxygen (DO) values [mg/L].
Exposure period [h]
|
Concentration [mg/L] |
|||||
|
Control |
7 |
13 |
25 |
50 |
100 |
0 (fresh) |
8.5 |
8.3 |
8.6 |
8.3 |
8.5 |
8.5 |
48 (aged) |
8.4 |
8.3 |
8.4 |
8.3 |
8.3 |
8.3 |
Table 4. Summary of temperature values [°C].
Exposure period [h]
|
Concentration [mg/L] |
|||||
|
Control |
7 |
13 |
25 |
50 |
100 |
0 |
21.2 |
21.2 |
21.2 |
21.2 |
21.2 |
21.2 |
24 |
21.7 |
21.7 |
21.7 |
21.7 |
21.7 |
21.7 |
48 |
21.7 |
21.7 |
21.7 |
21.7 |
21.7 |
21.7 |
REFERENCE TEST
Results with the reference item Potassium dichromate are summarized in Table 5. After 48 h, 100% immobilization was observed at 1.2 mg/L.
Table 5. Results with Potassium dichromate (positive control)
Exposure period |
EC50 value (95% confidence limits) |
NOEC |
24 h |
1.13 mg/L (0.85 – 2.2) |
- |
48 h |
0.84 mg/L (0.83 – 0.86) |
0.5 mg/L |
BIOLOGICAL RESULTS
Cumulative immobilization data is provided in Table 6 and the corresponding EC50 values are provided in Table 7.
Table 6. Cumulative immobilization.
Concentration |
Number immobilized |
% immobilisation |
||
[mg/L] |
24 h |
48 h |
24 h |
48 h |
0 |
0 |
0 |
0 |
0 |
7 |
0 |
0 |
0 |
0 |
13 |
0 |
0 |
0 |
0 |
25 |
1 |
2 |
5 |
10 |
50 |
0 |
0 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
Table 7. EC50 values.
Exposure time [h] |
EC50 value [mg/L] |
95% confidence limits [mg/L] |
24 |
> 100 |
not determinable |
48 |
> 100 |
not determinable |
Description of key information
No effects up to the limit of water solubility (nominal, OECD 202, D. magna)
Key value for chemical safety assessment
Additional information
One study is available, in which the short-term toxicity of Monoesters of C16 and C18 (branched and linear) fatty acids with decan-1-ol to aquatic invertebrates was assessed according to OECD guideline 202 and GLP in freshwater (key study).
In a static test, the model organism Daphnia magna was exposed to five nominal concentrations of 7, 13, 25, 50, and 100 mg/L for 48 h. No chemical analysis of test item concentrations was performed. For each nominal concentration the required amount of homogenized sample was added to the appropriate amount of dilution water in an aspirator. The aspirators were mixed for 20 – 24 h and then allowed to separate for 4 h. The aqueous phase, avoiding all settled and floating material, was drawn off and used for the test.
After 48 h, no significant immobilization was observed (max 10%), resulting in an EC50 (48 h) of > 100 mg/L and a NOEC (48 h) of ≥ 100 mg/L.
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