2,4-dichlorobenzoyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Two weeks observation after one administration of the substance via gavage

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichlorobenzoyl chloride

EC Number:

201-936-4

EC Name:

2,4-dichlorobenzoyl chloride

Cas Number:

89-75-8

Molecular formula:

C7H3Cl3O

IUPAC Name:

2,4-dichlorobenzoyl chloride

Constituent 2

Reference substance name:

Benzoylchloride, 2,4-dichloro-

IUPAC Name:

Benzoylchloride, 2,4-dichloro-

Test material form:

other: Liquid

Details on test material:

Name: 2,4-Dichlorbenzoylchlorid
Physical condition: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Male Rats: SPF-Rats (Wistar TNO W 74, Breeding: Winkelmann, Borchen)
Female Rats: SPF- Rats( Wistar TNO W 74, Breeding: Winkelmann, Borchen)
7/19h (12 h dark/light)
5 animals/cage
Room Temperatur: 22(+/- 1.5 °C)
Humidity: 60 (+/- 5 %)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The substance was concentrated with a metal tube

Doses:

Male:[2.0,3.10,3.5,3.8,4.0,4.5,5.0 ]ml/kg once orally administered
Female: [ 1, 2, 2.5, 3.1,4] ml/kg once orally administered

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

The male rats were 9 weeks old and the female rats were 14 weeks old. The rats were kept in a makrolon cage typ III with each 5 animals per cage( sex specific).The animals were fed with Altromin R 1324 (Altromin GmbH, Lage) and tap water ad libitum. Administration of 2,4-Dichlorobenzoyl chloride once.

Statistics:

Probit-Analysis

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

3.78 mL/kg bw

95% CL:

>= 3.55 - <= 4

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

2.58 mL/kg bw

95% CL:

>= 2.29 - <= 2.82

Clinical signs:

other: Lethargy, piloerection,sedation, Body weight loss

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 for acute oral toxicity is 2.58 ml/kg bw in female Wistar and 3.78 ml/kg in male rats.