Registration Dossier
Registration Dossier
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EC number: 612-381-6 | CAS number: 61789-91-1
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- no defined IUPAC name
- EC Number:
- 612-381-6
- Cas Number:
- 61789-91-1
- Molecular formula:
- no molecular structure defined
- IUPAC Name:
- no defined IUPAC name
- Test material form:
- semi-solid (amorphous): gel
- Details on test material:
- Components Floraesters 30
Wax Ester ---
36:2 <2
38:0 <1
38:1 2 - 4
38:2 2 - 4
40:0 2 - 4
40:1 14 - 18
40:2 17 - 21
42:0 2 - 4
42:1 14 - 18
42:2 17 - 21
44:0 <2
44:1 4 - 7
44:2 4 - 7
46:2 <3
Iodine Value** 57 - 61
Mono-ester content*** 40 - 47
Constituent 1
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 937 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Applicant's summary and conclusion
- Conclusions:
- The concentration dependent inhibition of respiration according to OECD 209 was determined for both the base and the test substance formulation. A dose response for a range of test substance concentration was conducted. The measured values were then graphed and a curve fit was applied to determine test substance concentration at which the measured sludge repsiration was reduced by 50% (EC50).
The graphs showing the plotted values are fit by non-linea regression and provide both the mean statistics for each measure data point and the 95% confidence intervals for the respective curve fit.
The EC50 value determined for the test substance formulation was 3.468 Log10(EC50) or 2937 ppm. The EC50 value determined for the base was 4.149 Log10(EC50) or 14092 ppm. The test result graphs show the independent determinations for the base formulation alone and with the addition of 10% Floraester 30 at each measured concentration of the base formulation. The reference substance (3,5 dichlorophenol) demonstrated a reduction from 29.3 O2 ppm/g dwt/hr to 4.9 O2 ppm/g dwt/hr at a concentration of 20 ppm, validating the sensitvity of the sludge respiration to the positive reference substance.
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