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EC number: 265-777-2 | CAS number: 65442-31-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13th December 2018 to 26 February 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- Version from 23th March 2006
- Deviations:
- yes
- Remarks:
- The deviation can be considered as uncritical
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name: 6-sec-butylquinoline
- Purity by GLC: > 99.2%
- Lot number: 6SBQ5/2017
- Molecular formula: C13H15N
- Molecular weight: 185.26955 g/mol - Analytical monitoring:
- yes
- Details on sampling:
- Samples were diluted with an equal amount of acetonitrile (dilution factor 2). A second dilution step was done for the sample with higher concentrations (10 * LOQ) using acetonitrile / medium 50/50 (% v/v) in a ratio 1:1.
- Vehicle:
- no
- Details on test solutions:
- Date: 08. – 11. Jan. 2019
Treatments tested: 0.1 / 0.32 / 1 / 3.2 / 10 mg/L nominal concentration (The concentrations to be tested are based on non GLP pre-tests) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- SPECIFICATION
Unicellular freshwater green alga.
Genus: Desmodesmus
Species: subspicatus
SAG Strain Number: 86.81
Taxonomic position: Chlorophyta - Chlorophyceae
ORIGIN AND CULTURE
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not measured
- Test temperature:
- 21.0 to 24.0 °C (the temperature fluctuates by more than ± 2 °C. As all validity criteria were met and exponential growth was observed in the blank control this was stated as uncritical)
- pH:
- 7.5 to 9.1
- Dissolved oxygen:
- not measured
- Salinity:
- not measured
- Conductivity:
- not measured
- Nominal and measured concentrations:
- At the start and at the end of the test and every 24 hours, the content of the test item in the test solutions was determined using HPLC-UV.
The test item was not stable during the test. The measured concentrations lay between 89 % and 100 % of the nominal concentrations at the beginning of the test and between 48 % and 51 % of the nominal concentrations at the end of the test. In the lowest concen-tration the test item was no longer quantifiable after 24 hours incubation.
Therefore, the determination of the results was based on the geometric mean of the measured concentrations.
The lowest concentration is not necessary for evaluation, since the next higher concentration does not show any inhibition either. Therefore, the lowest concentration is not used for evaluation. - Details on test conditions:
- TEST SYSTEM
- Test vessel: closed glass flasks total volume 65 mL
- Type (delete if not applicable): closed
- Aeration: no
- Initial cells density: 2580 cell/mL
- Control end cells density: 174533 cell/mL (72h)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201 medium)
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: pH was measured at the beginning of the test and at the end for the control and all test concentrations.
OTHER TEST CONDITIONS
- Sterile test conditions: yes (all used solution were sterilized before use)
- Adjustment of pH: no
- Photoperiod: continuous lighting
- Light intensity and quality: 5000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Test concentrations: 0.1 / 0.32 / 1 / 3.2 / 10 mg/L nominal concentration
- Results used to determine the conditions for the definitive study: The concentrations to be tested are based on non GLP pre-tests - Reference substance (positive control):
- yes
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software (Microsoft Ex-cel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for growth rate inhibition (72h-EC50) was 1.82 mg/l based on nominal concentrations. The EC10 for growth rate inhibition was 0.30 mg/L based on nominal concentrations.
- Executive summary:
The study was performed using 5 concentrations ranging from 0.1 to 10 mg/L. Incubation time (test systemDesmodesmus subspicatus)was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1]were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
1 – 10 mg/L (nominal concentration)
At the start and at the end of the test and every 24 hours, the content of the test item in the test solutions was determined using HPLC-UV.
The test item was not stable during the test. The measured concentrations lay between 89 % and 100 % of the nominal concentrations at the beginning of the test and between 48 % and 51 % of the nominal concentrations at the end of the test. In the lowest concentration the test item was no longer quantifiable after 24 hours incubation.
Therefore, the determination of the results was based on the geometric mean of the measured concentrations.
The lowest concentration is not necessary for evaluation, since the next higher concentration does not show any inhibition either. Therefore, the lowest concentration is not used for evaluation.
The 72h-EC50values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
The following results for the test item6-sec-butylquinolinewere determined:
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
0.19 mg/L
0.59 mg/L
0.30 mg/L
1.82 mg/L
Yield
0.19 mg/L
0.59 mg/L
0.51 mg/L
0.57 mg/L
Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10which is read from the graph toxicity vs. concentration may lie lower.
Reference
Description of key information
The EC50 for growth rate inhibition (72h-EC50) was 1.82 mg/l based on nominal concentrations. The EC10 for growth rate inhibition was 0.30 mg/L based on nominal concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.82 mg/L
- EC10 or NOEC for freshwater algae:
- 0.3 mg/L
Additional information
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
0.19 mg/L |
0.59 mg/L |
0.30 mg/L |
1.82 mg/L |
Yield |
0.19 mg/L |
0.59 mg/L |
0.51 mg/L |
0.57 mg/L |
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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