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EC number: 268-040-3 | CAS number: 67990-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04.10.2018 - 13.11.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- TOXI-COOP ZRT., Arácsi út 97., 8230 Balatonfüred, Hungary
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of the test item concentrations, four replicate samples (5 mL per replicate) were taken from the test solution (100 mg/L) and from the control at the start and at the end of the test. The analysis was performed by the analytical laboratory of TOXI-COOP ZRT.
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by mechanical dispersion without using of any solubilising agent. An amount of 0.03 g test item was dissolved in 300 mL test medium (ISO Medium) resulting a nominal concentration of 100 mg/L. After the formulation procedure test animals were immediately introduced into the test solution or control.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Strain: Straus
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection), 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Feeding during test: no
ACCLIMATION
- Acclimation period: 48h
- Acclimation conditions: same as test conditions
- Type and amount of food: concentrated algal suspension of Raphidocelis subcapitata during the holding
- Health during acclimation: no mortality - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 20.0 - 20.3°C
- pH:
- 7.57 - 7.83
- Dissolved oxygen:
- 7.28 - 7.41 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Measured concentration: 106.2 mg/L (start); 109.6 mg/L (end) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Material, size, fill volume: glass, 50 mL, 40 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM PARAMETERS
- Preparation of dilution water: reconstituted water (ISO medium) according to OECD 202
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours of light and 8 hours dark cycle
- Light intensity: 1171 lux
EFFECT PARAMETERS MEASURED:
Each test vessel was observed for immobilized Daphnids at 24 and 48 hours after the beginning of the test.
The temperature, dissolved oxygen concentration and pH were measured in the test item treated and the control group at the start (before test solutions had been distributed into the test vessels) and in each test vessel at the end of the test. Additionally, the ambient temperature was measured continuously using a min/max thermometer in the climate chamber.
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10.0 and 100.0 mg/L
- Results used to determine the conditions for the definitive study: Yes, during range finding study no immobility was observed. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- The date of the last study (Study Number: 392-202-4076) with reference item Potassium dichromate was: 20 – 21 September 2018.
The 24-h EC50: 1.48 mg/L, (95 % confidence limits: 1.21 – 1.71 mg/L). - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of the test item on Daphnia magna was determined to be >100 mg/L.
- Executive summary:
The test item was evaluated for acute toxicity on Daphnia as per the OECD Guideline No. 202, “Daphina sp., Acute Immobilisation Test”. The acute toxicity study of the test item on Daphnia was performed to determine the 48 hours Effective Concentration (EC50) and to determine Lowest Observed Effect Concentration (LOEC) and No Observed Effect Concentration (NOEC). Daphnids were exposed to the test item by aqueous exposure under static condition. Daphnids were observed at 24 and 48 hours for signs of toxicity and immobility. Based on the results of the range finding study, a limit test was conducted as main study at the test concentration of 100.0 mg/L in the test medium along with a control. No clinical signs of toxicity were observed in control and in the tested concentration of 100 mg/L during the 48 hour observation period. During the main study, a sample from the test concentration was collected at the start and the end of the test and analyzed. Results showed that the test concentrations were within the acceptable range of ± 20% to the nominal concentrations. Therefore the results are based on the nominal concentration. Based on the results of the experiment it can be concluded that after 48 hours the EC50 of the test item on Daphnia is >100 mg/L. The No Observed Effect Concentration (NOEC) over the 48 hour exposure period is 100 mg/L, the and Lowest Observed Effect Concentration (LOEC) is >100 mg/L.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
- Justification for type of information:
- Please refer to QMRF and QPRF in the section "Attached justification"
- Qualifier:
- according to guideline
- Guideline:
- other: REACH guidance on QSAR R.6
- Principles of method if other than guideline:
- Calculation of acute toxicity in dapnia. Software used: ECOSAR 1.11 (EPISUITE) for chemical class "Neutral Organics-acid".
- GLP compliance:
- no
- Remarks:
- Not applicable for QSAR calculation
- Specific details on test material used for the study:
- SMILES: CCCCOCCOCC(=O)O
- Analytical monitoring:
- not required
- Test organisms (species):
- Daphnia sp.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15 293.602 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Using ECOSAR v1.11 the short-term toxicity value for daphnia was calculated to be 15293.602 mg/L. The substance is within the applicability domain of the model.
Referenceopen allclose all
ECOSAR v1.11 Class-specific Estimations
Not Related to an Existing ECOSAR Class Definition:
Estimates provided below use the Neutral Organics QSAR equations which represent baseline toxicity potential (minimum toxicity) assuming a simple non-polar narcosis model. Without empirical data on structurally similar chemicals, it is uncertain if this substance will present significantly higher toxicity above baseline estimates.
NOTE: METAL ([Na], [Li] or [K]) HAS BEEN REMOVED FOR PROPER ESTIMATION.
SALTS SHOULD BE ENTERED IN ECOSAR AS THE NEUTRAL NON-SALT FORM OF THE MOLECULE CORRESPONDING TO EITHER THE FREE BASE OR CONJUGATE ACID.
-> Acid moeity found: Predicted values multiplied by 10
Predicted ECOSAR Class |
Organism |
Duration |
End Pt |
mg/L (ppm) |
Neutral Organics-acid |
Daphnid |
48-hr |
LC50 |
15293.602 |
Description of key information
The 48-hour EC50 of the test item on Daphnia magna was determined to be >100 mg/L.
Using ECOSAR v1.11 the short-term toxicity LC50 value for daphnia was calculated to be 15293.602 mg/L. The substance is within the applicability domain of the model.
Key value for chemical safety assessment
Additional information
The test item was evaluated for acute toxicity on Daphnia as per the OECD Guideline No. 202, “Daphina sp., Acute Immobilisation Test”. The acute toxicity study of the test item on Daphnia was performed to determine the 48 hours Effective Concentration (EC50) and to determine Lowest Observed Effect Concentration (LOEC) and No Observed Effect Concentration (NOEC). Daphnids were exposed to the test item by aqueous exposure under static condition. Daphnids were observed at 24 and 48 hours for signs of toxicity and immobility. Based on the results of the range finding study, a limit test was conducted as main study at the test concentration of 100.0 mg/L in the test medium along with a control. No clinical signs of toxicity were observed in control and in the tested concentration of 100 mg/L during the 48 hour observation period. During the main study, a sample from the test concentration was collected at the start and the end of the test and analyzed. Results showed that the test concentrations were within the acceptable range of ± 20% to the nominal concentrations. Therefore the results are based on the nominal concentrations. Based on the results of the experiment it can be concluded that after 48 hours the EC50 of the test item on Daphnia is >100 mg/L. The No Observed Effect Concentration (NOEC) over the 48 hour exposure period is 100 mg/L, the and Lowest Observed Effect Concentration (LOEC) is >100 mg/L.
The short-term toxicity to aquatic invertebrates of the test substance was calculated using ECOSAR v.1.11 as part of EPISuite v4.11 from US Environmental Protection Agency. Using ECOSAR v1.11 the short-term toxicity LC50 value for daphnia was calculated to be 15293.602 mg/L (EPI Suite, 2014).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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