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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24th January 2019 - 21st February 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2020
Report date:
2020

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -I: Aqueous Exposure Bioconcentration Fish Test
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of 1,2,3,4,4a,5,6,7-octahydro-2,5,5-trimethyl-2-naphthol and 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one and 4-(2,6,6-trimethyl-2-cyclohexen-1-yl)butan-2-one
EC Number:
915-318-3
Cas Number:
41199-19-3, 17283-81-7 and 31499-72-6
IUPAC Name:
Reaction mass of 1,2,3,4,4a,5,6,7-octahydro-2,5,5-trimethyl-2-naphthol and 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one and 4-(2,6,6-trimethyl-2-cyclohexen-1-yl)butan-2-one
Test material form:
liquid

Sampling and analysis

Details on sampling:
The test sample and HCO-40 were dissolved in N,Ndimethylformamide to prepare stock solution. This tsock solution was diluted appropriately with dilution water to prepare the test solution.

Test solutions

Vehicle:
yes
Remarks:
feed
Details on preparation of test solutions, spiked fish food or sediment:
Feed: Feed for colored carp
Composition: Proteins content 230%
Lipid content 24.0%

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Common carp
- Age at study initiation: Yearling fish
- Length at study initiation: 6.2- 6.7 cm
- Weight at study: The minimum fish weight at the beginning of uptake phase was more than two thirds of the maximum one.
- Feeding during test
- Food type: Feed for colored carp
- Amount: 2% of total body weight
- Frequency: daily

ACCLIMATION
- Acclimation period: 90 days
- Acclimation conditions: 25+/-2°C

Study design

Route of exposure:
feed
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d

Test conditions

Hardness:
1 8.8 mgCaCO3/L
Test temperature:
25.0-25.2°C
pH:
7.6-7.5
Dissolved oxygen:
7.0-7.6 mg/L
TOC:
9.41-10.2 mgCL
Details on test conditions:
Aeration No aeration
Time of irradiation with light: 14 hours light 11 0 hours dark (artificial light of white fluo-t lamp)
Number of fish (at the beginning of uptake phase)
Levels 1 and 2: 32 fish/ level
Control 1: 6 fish/ level
Nominal and measured concentrations:
0.1 and 0.01 mg/L
Reference substance (positive control):
no

Results and discussion

Lipid contentopen allclose all
Lipid content:
4.22 %
Time point:
start of exposure
Lipid content:
4.89 %
Time point:
end of exposure
Bioaccumulation factoropen allclose all
Key result
Conc. / dose:
0.1 mg/L
Temp.:
25 °C
pH:
7
Type:
BCF
Value:
< 28 L/kg
Basis:
total lipid content
Calculation basis:
steady state
Key result
Conc. / dose:
0.01 mg/L
Temp.:
25 °C
pH:
7
Type:
BCF
Basis:
total lipid content
Calculation basis:
steady state
Remarks on result:
not determinable
Rate constants
Key result
Rate constant:
growth rate constant (d-1)
Value:
0.016

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The bioconcentration factor for a 0.1 mg/L concentration was 28 L/Kg at steady state and was not determinable at 0.01 mg/L concentration.