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EC number: 235-166-5 | CAS number: 12108-13-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented publication. No guideline available for this type of study nor is this route considered for classification purposes.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 982
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- no
- Remarks:
- Study conducted prior to GLP guidelines
- Limit test:
- no
Test material
- Reference substance name:
- Tricarbonyl(methylcyclopentadienyl)manganese
- EC Number:
- 235-166-5
- EC Name:
- Tricarbonyl(methylcyclopentadienyl)manganese
- Cas Number:
- 12108-13-3
- Molecular formula:
- C9H7MnO3
- IUPAC Name:
- tricarbonyl(methyl-η5-cyclopentadienyl)manganese
- Details on test material:
- Name of test material: methylcyclopentadienyl manganese tricarbonyl
Constituent 1
Test animals
- Species:
- other: mouse and rat
- Strain:
- other: BALB/c mice and CD/CR rats
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: bred in the lab facilities (mice); Charles River Laboratories (rats)
- Age at study initiation: 8 to 12 weeks old (mice); 3 to 6 months old (rats)
- Weight at study initiation: 20 to 25g (mice); 250 to 300 g (rats)
- Fasting period before study: no
- Diet: ad libitum
- Water: ad libitum
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- corn oil
- Details on exposure:
- mmt was dissolved in corn oil and administered by ip injection to rats at 5 mg/kg and mice at 120 mg/kg.
- Doses:
- see details on exposure
- No. of animals per sex per dose:
- 8 to 10 mice and 4 to 6 rats.
- Control animals:
- yes
- Details on study design:
- Immediately after each treatment, half of the treated animals and half of the controls were placed within their cages for 6 days, inside a 450-liter plastic chamber which was continuously being ventilated with 80% O2 at an average flow rate of 15 liters/min. The temperate within the chamber remained at 24 to 25 ° C with a relative humidity of 55 to 65%. Oxygen concentrations within the chamber was periodically monitored with an oxygen meter and stayed within ± 3% of the desired concentration. Control animals were kept in room air. After 6 days, the animals were removed from the O2 and kept in room air. Animals were killed for histologic examination and for measurement of lung hydroxyproline levels 3 weeks after administration of the pneumotoxic agent.
- Statistics:
- Data were calculated as mean ± SE, and comparisons between groups were made with Student's t test. A p value of 0.05 or less was considered to be significant. Where appropriate, one-way analysis of variance was performed on data followed by comparisons of the means by the honestly significant difference procedure, values were considered significant at 5% level.
Results and discussion
- Mortality:
- -
- Clinical signs:
- -
- Body weight:
- -
- Gross pathology:
- Exposure of Controls to 80% 02
The exposure to 80% O2 alone produced a statistically significant increase (13% increase in total lung hydroxyproline over controls in mice) once in more than 20 different experiments. No effect of 80% O2 alone was found in rats.
Mice or rats exposed to O2 for 6 days, followed by a 2- week recovery period, did not show any significant histopathologic changes in the lungs..
Methylcyclopentadienyl manganese tricarbonyl (mmt) exposure
Three weeks after mmt treatment alone , total lung hydroxyproline was only slightly increased as compared to controls; the difference was statistically not significant.
However, in mice treated with mmt and exposed to 80% 02, a significant increase in the hydroxyproline content was found (Fig. 1A). Histopathological examination showed interstitial thickening characterized by mild hypercellularity and fibrosis, localized primarily around terminal bronchioles and alveolar ducts. It was determined that combined treatment of mmt and O2 produced collagen accumulation and interstitial fibrosis by biochemical and histopathologic criteria. A significant increase in total lung hydroxyproline was found biochemically (fig. 1A).
In rats, oxygen exposure failed to further increase lung collagen, contrary to what had been found in mice. On histopathologic examination, lungs of rats exposed to both mmt and 80% O2 were indistinguishable from those animals treated with mmt alone. - Other findings:
- -
Applicant's summary and conclusion
- Conclusions:
- In the present study, more than 80% of the treated animals survived the 3 weeks post-exposure. In mice, the development of fibrosis was significantly enhanced if animals treated with mmt were exposed to 80% O2 immediately following the acute lung injury. Mice treated with mmt alone did not produce a significant increase in hydroxyproline, however when preceded by exposure to 80% oxygen, the hydroxyproline content increased significantly in the lung. In rats, mmt alone caused an increase in hydroxyproline content, and in contrast with the mice, the oxygen post-exposure did not produce any changes of hydroxyproline levels in the rat lung.
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