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EC number: 289-969-0 | CAS number: 90046-02-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Juniperus oxycedrus, Cupressaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 September 2019 - 02 October 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): A sample of activated sludge was taken from the aeration tank of Sewage Treatment Plant ”Czajka” , Warsaw, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was aerated for 7 days, at the test temperature of about 22 °C, until application. A sample was withdrawn just before use for the determination of the dry weight of the suspended solids. Before application the sludge was washed in a mineral medium.
- Concentration of sludge: 3.91 g suspended solids/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Remarks:
- (50.0 mg/l of organic carbon)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 10ml of solution (A) in 800 ml water, plus 1 ml solutions (B), (C), (D) and dilution water up to 1 L. The following stock solutions were used, prepared with analytical grade reagents: Solution (A) contains: 8.50 g monopotassium dihydrogen orthophosphate (KH2PO4), 21.75 g dipotassium monohydrogen orthophosphate (K2HPO4), 33.40 g disodium monohydrogen orthophosphate dihydrate (Na2HPO4·2H2O), 0.50 g ammonium chloride (NH4Cl), in 1 L water. Solution (B) contains: 27.50 g calcium chloride, anhidrous (CaCl2) in 1L water. Solution (C) contains: 22.50 g magnesium sulphate heptahydrate (MgSO4·7H2O) in 1L water. Solution (D) contains: 0.25 g iron(III) chloride hexahydrate (FeCl3·6H2O) in 1L water.
- The water used is double-distilled, containing 3.1±0.4 mg/L of organic carbon (only slightly higher than 10% of the organic carbon content introduced by the test item), checked by DOC analysis using spectrophotometer Hach DR 3900 and Hach-Lange reagents.
- Test temperature: 22 ± 1ºC
- pH: 7.4 ± 0.2
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
- Volume of test solution in flask, V: 0.164 L
TEST SYSTEM
- Number of culture flasks/concentration: triplicates were used, flasks #7, 8, 9 containing test item (100 mg/l) and inoculum (30mg/L SS).
- Measuring equipment: O2 uptake was measured by WTW OxiTop OC 110 repirometer.
- Details of trap for CO2 and volatile organics if used: potassium hydroxide solution.
SAMPLING
- Sampling frequency: O2 uptake data were read out every 112 min during the 28 day test (40 320 min that is 360 readings) and were recorded and stored in the measuring heads of the sample bottles.
- Sampling method: Readings from apparatus (WTW OxiTop OC 110 repirometer).
CONTROL AND BLANK SYSTEM
- Inoculum blank: flasks #1, 2, 3, containing only inoculum 30mg/L SS.
- Procedure control: flasks #4, 5, 6, containing reference item (sodium acetate 100 mg/l) and inoculum 30mg/L SS.
- Toxicity control: flasks #10, 11, 12, containing test item, reference item at the same concentrations as in the individual solutions and inoculum 30mg/L SS.
- Other: correction for interference by nitrification:
-Flasks 0a1, 0a2: Test suspension containing test item (100 mg/L) and inoculum at start.
-Flasks 0tox1/1, 0tox1/2: Test suspension containing test item and reference item at the same concentrations as in the individual solutions and inoculum at start.
STATISTICAL METHODS: The calculations and the graphs were performed using SigmaPlot 9.0 software of SYSTAT Software, Inc., USA purchased from GAMBIT CoiS Ltd, Poland. - Reference substance:
- acetic acid, sodium salt
- Remarks:
- CAS No: 127-09-3, purity p.a. 99.7%, source: CHEMPUR, Piekary Śląskie, Polska.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 57.5
- Sampling time:
- 28 d
- Details on results:
- -The biodegradability attained 57.5% within 28 days. The pass level for ready biodegradability is 60% of ThOD (or COD).
-The reference item reached 96.9% of biodegradation and the level for ready biodegradability by 4 days
-In the toxicity test the biodegradation was equal to 52.0% in 14 days
-The oxygen uptake of the inoculum blank was equal to 35.8 mg/L in 28 days
-The pH values of flasks were in the range of 7.56-7.64 - Results with reference substance:
- The reference item reached 96.9% of biodegradation and the level for ready biodegradability by 4 days (>60% reached before day 14).
- Validity criteria fulfilled:
- yes
- Remarks:
- See "overall remarks".
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the ready aerobic biodegradability test, the test item attained 57.5% biodegradation thus it can not be classed as readily biodegradable.
- Executive summary:
A ready biodegradability test in an aerobic aqueous medium with manometric respirometry method was performed on the test item, according to OECD 301F / EC C.4 – D manometric respirometry methods, following GLP principles. 100 mg/l of test item was inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at constant temperature (22 ± 0.40 ºC) for 28 days. A blank test, a procedure test with reference substance (sodium acetate) and a toxicity test were run in parallel. Also, as the test item is a nitrogen-containing substance the obtained results for oxygen uptake were corrected for interference by nitrification using additional controls in the test item and toxicity tests. For assessing the biodegradation level (%), COD determination of the test item was used instead of ThOD because of the substance is an UVCB substance. In the toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on COD) attained 52.0%. Thus, the test item can be considered as not inhibitory. All validity criteria were fulfilled. The biodegradability of the test item attained 57.5 % within 28 days. The pass level for ready biodegradability is 60% of ThOD (or COD). Thus, the test item can not be classed as readily biodegradable.
Reference
Table 2. Sample oxygen uptake: biodegradability.
Time, days |
1 |
3 |
5 |
7 |
9 |
12 |
14 |
16 |
18 |
21 |
23 |
25 |
28 |
|
Test item O2uptake, mg/L |
a1 |
6.8 |
40.0 |
51.7 |
62.7 |
70.4 |
82.7 |
87.7 |
93.5 |
99.1 |
107.8 |
114.5 |
121.4 |
129.1 |
a2 |
5.5 |
40.0 |
48.3 |
59.6 |
69.1 |
80.5 |
84.9 |
90.5 |
95.3 |
104.0 |
110.8 |
115.6 |
124.1 |
|
a3 |
6.1 |
40.7 |
51.3 |
61.4 |
70.9 |
81.3 |
87.9 |
93.3 |
99.1 |
105.0 |
113.5 |
118.6 |
125.4 |
|
am. avg |
6.1 |
40.2 |
50.4 |
61.2 |
70.1 |
81.5 |
86.8 |
92.4 |
97.8 |
105.6 |
112.9 |
118.5 |
126.2 |
|
Time, days |
1 |
3 |
5 |
7 |
9 |
12 |
14 |
16 |
18 |
21 |
23 |
25 |
28 |
|
Blank test O2uptake. mg/L |
b1 |
9.3 |
14.1 |
16.2 |
16.9 |
20.6 |
24.0 |
25.2 |
25.8 |
28.3 |
30.5 |
33.3 |
34.8 |
37.9 |
b2 |
8.3 |
13.3 |
15.6 |
18.9 |
20.2 |
23.7 |
25.4 |
25.0 |
26.0 |
27.3 |
29.5 |
31.8 |
33.9 |
|
b3 |
6.7 |
12.7 |
16.1 |
19.0 |
19.3 |
24.8 |
25.1 |
27.2 |
27.0 |
30.1 |
31.4 |
32.7 |
35.6 |
|
bmavg |
8.1 |
13.4 |
15.9 |
18.3 |
20.1 |
24.2 |
25.2 |
26.0 |
27.1 |
29.3 |
31.4 |
33.1 |
35.8 |
|
Reference item O2uptake. mg/L |
w1 |
18.3 |
58.7 |
70.6 |
77.2 |
85.2 |
93.2 |
98.3 |
98.5 |
101.1 |
106.9 |
108.8 |
110.1 |
112.9 |
w2 |
21.7 |
60.4 |
72.7 |
80.2 |
87.9 |
94.0 |
99.1 |
98.6 |
101.0 |
103.3 |
104.5 |
107.1 |
109.9 |
|
w3 |
19.1 |
60.9 |
71.8 |
80.8 |
87.8 |
93.8 |
95.7 |
98.5 |
100.0 |
101.1 |
104.0 |
107.1 |
109.6 |
|
wm. avg |
19.7 |
60.0 |
71.7 |
79.4 |
87.0 |
93.7 |
97.7 |
98.5 |
100.7 |
103.8 |
105.8 |
108.1 |
110.8 |
|
Toxicity control O2uptake mg/L |
tox1 |
12.2 |
88.3 |
117.7 |
132.1 |
141.8 |
155.8 |
162.2 |
168.2 |
174.1 |
181.0 |
189.5 |
194.7 |
204.5 |
tox2 |
10.9 |
84.4 |
103.1 |
123.7 |
132.4 |
146.1 |
154.7 |
-- |
-- |
-- |
-- |
-- |
-- |
|
tox3 |
15.6 |
86.6 |
115.9 |
128.5 |
137.0 |
153.8 |
161.9 |
-- |
-- |
-- |
-- |
-- |
-- |
|
tox2-3avg |
13.3 |
85.5 |
109.5 |
126.1 |
134.7 |
150.0 |
158.3 |
-- |
-- |
-- |
-- |
-- |
-- |
|
Corrected test item O2uptake, mg/L |
a1- bm |
-1.3 |
26.6 |
35.7 |
44.4 |
50.4 |
58.6 |
62.5 |
67.5 |
71.9 |
78.5 |
83.1 |
88.3 |
93.3 |
a2- bm |
-2.6 |
26.6 |
32.3 |
41.3 |
49.0 |
56.3 |
59.7 |
64.5 |
68.2 |
74.7 |
79.4 |
82.5 |
88.3 |
|
a3- bm |
-2.0 |
27.3 |
35.3 |
43.1 |
50.8 |
57.1 |
62.7 |
67.3 |
72.0 |
75.7 |
82.1 |
85.5 |
89.6 |
|
Reference item %degradation, % ThOD = 0.78 mgO2/mg C = 100 mg/L |
R1(w1) |
13.1 |
58.2 |
70.1 |
75.6 |
83.5 |
88.5 |
93.6 |
93.0 |
94.9 |
99.5 |
99.2 |
98.7 |
98.9 |
R2(w2) |
17.4 |
60.2 |
72.8 |
79.5 |
86.9 |
89.5 |
94.8 |
93.0 |
94.7 |
94.9 |
93.7 |
94.9 |
95.0 |
|
R3(w3) |
14.1 |
60.9 |
71.7 |
80.2 |
86.9 |
89.2 |
90.3 |
93.0 |
93.4 |
92.1 |
93.1 |
94.9 |
94.6 |
|
Rrefavg |
15.3 |
59.2 |
71.4 |
77.5 |
85.2 |
89.0 |
94.2 |
93.0 |
94.8 |
97.2 |
96.5 |
96.8 |
96.9 |
|
Test item% degradation COD = 1.60 mgO2/mg C = 100 mg/L |
R1(a1) |
0.0 |
16.6 |
22.3 |
27.8 |
31.5 |
36.6 |
39.0 |
42.2 |
45.0 |
49.0 |
51.9 |
55.2 |
58.3 |
R2(a2) |
0.0 |
16.6 |
20.2 |
25.8 |
30.6 |
35.2 |
37.3 |
40.3 |
42.6 |
46.7 |
49.6 |
51.6 |
55.2 |
|
R3(a3) |
0.0 |
17.1 |
22.1 |
27.0 |
31.8 |
35.7 |
39.2 |
42.0 |
45.0 |
47.3 |
51.3 |
53.4 |
56.0 |
|
R1-3avg |
0.0 |
16.8 |
21.5 |
26.8 |
31.3 |
35.8 |
38.5 |
41.5 |
44.2 |
47.7 |
51.0 |
53.4 |
56.5 |
|
Toxicity test % degradation
|
R1tox1 |
1.7 |
31.5 |
42.7 |
47.8 |
51.1 |
55.3 |
57.5 |
59.8 |
61.8 |
63.7 |
66.4 |
67.9 |
70.9 |
R2tox2 |
1.2 |
29.9 |
36.6 |
44.3 |
47.2 |
51.2 |
54.4 |
-- |
-- |
-- |
-- |
-- |
-- |
|
R3tox3 |
3.2 |
30.8 |
42.0 |
46.3 |
49.1 |
54.5 |
57.4 |
-- |
-- |
-- |
-- |
-- |
-- |
|
R2-3avg |
2.0 |
30.7 |
40.5 |
46.1 |
49.2 |
53.7 |
56.5 |
-- |
-- |
-- |
-- |
-- |
-- |
Table 3. Correction for oxygen uptake for interference by nitrification. Test item
days |
0 |
28 |
difference |
|||
1) Concentration of nitrate (mg N-NO3/L) |
Flask #0a1 |
Flask #0a2 |
Flask #7 |
Flask #8 |
Flask #9 |
-0.366 |
0.809 |
0.793 |
0.426 |
0.404 |
0.476 |
||
Avg: 0.801 ± 0.011 |
Avg: 0.435 ± 0.037 |
|||||
2) Oxygen equivalent (4.57× N-NO3) (mg/L) |
-1.673 |
|||||
3) concentration of nitrite (mg N-NO2/L) |
Flask #0a1 |
Flask #0a2 |
Flask #7 |
Flask #8 |
Flask #9 |
difference |
0.006 |
0.007 |
0.008 |
0.005 |
0.009 |
0.001 |
|
Avg: 0.006 ± 0.001 |
Avg: 0.007 ± 0.002 |
|||||
4) Oxygen equivalent (3.43×N-NO2) (mg/L) |
0.003 |
|||||
5) total oxygen equivalent 2) + 4) |
-1.670 |
Table 4. Correction for oxygen uptake for interference by nitrification. Toxicity test
days |
0 |
14 |
difference |
||
1) Concentration of nitrate (mg N-NO3/L) |
Flask #0tox1/1 |
Flask #0tox1/2 |
Flask #11 |
Flask #12 |
-0.257 |
0.844 |
0.830 |
0.513 |
0.647 |
||
0.837 ± 0.01 |
0.580 ± 0.095 |
||||
2) Oxygen equivalent (4.57× N-NO3) (mg/L) |
-1.174 |
||||
3) concentration of nitrite (mg N-NO2/L) |
Flask #0tox1/1 |
Flask #0tox1/2 |
Flask #20 |
Flask #21 |
difference |
0.006 |
0.004 |
0.007 |
0.015 |
0.006 |
|
0.005 ± 0.001 |
0.011 ± 0.006 |
||||
4) Oxygen equivalent (3.43×N-NO2) (mg/L) |
0.021 |
||||
5) total oxygen equivalent 2) + 4) |
-1.153 |
Table 5. The pH values of test flasks (no adjustment of pH was conducted).
flask # |
7 |
8 |
9 |
1 |
2 |
3 |
4 |
5 |
6 |
10 |
11 |
12 |
Test item |
Inoculum blank |
Reference item |
Toxicity test |
|||||||||
initial |
7.57 |
7.64 |
7.62 |
7.62 |
7.62 |
7.62 |
7.54 |
7.60 |
7.60 |
7.66 |
7.59 |
7.59 |
final |
7.61 |
7.56 |
7.59 |
7.23 |
7.47 |
7.40 |
8.55 |
8.61 |
8.63 |
8.43 |
8.40 |
8.40 |
Description of key information
Key study: Test method according to OECD 301F / EU C.4 (Manometric respirometry methods), GLP study. The test item attained 57.5% biodegradation. Thus, the test item can not be classed as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.