Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, (1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl derivs.

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Test substance direclty added to test medium.

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Origin: activated sludge from municipal wastewater treatment plant.
- Preparation of inoculum: inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24 h. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test.

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

20 +/- 2°C

pH:

pH-value after correction: 7.1 - 7.2

Nominal and measured concentrations:

- Concentration tested (nominal): 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessels: Erlenmeyer vessel (nominal volume 250 ml)
- Test volume: 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20 ca. 2.7 mg/L
EC50 ca. 9.1 mg/L

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

non-GLP study

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

Draft

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Kupferphthalocyanin,
- Analytical purity: 99.3 %

Analytical monitoring:

no

Details on test solutions:

Substance is hardly soluble in water and were directly added without pretreatment.

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: Activated sludge from a laboratory wastewater plant treating municipal sewage.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

20 - 25 °C

Nominal and measured concentrations:

Nominal test concentration 500 and 1000 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 750 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Nominal test concentration [mg/L]	Respiration rate [mg/l*h]	Respiration inhibition [%]	Respiration promotion [%]
500	30	0	7
1000	16	43	0

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to attached justification.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 750 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.

Additional information

There are no studies available that assess the acute toxicity of the test substance to microorganisms. However, as the test substance belongs to the substance group of Phthalocyanines whose group members resemble in their chemical structure as well as in their phys-chem properties, a read-across approach was used to cover this endpoint.

Phtalocyanines were proofed to be not toxic to microorganisms by several studies. The changes in the respiration rate of activated sludge in presence of CAS 59160-79-1 were investigated in a GLP study according to OECD guideline 209 [BASF AG 1999]. At test termination after 30 minutes an EC20 > 1000 mg/L (nominal) was determined.

The effect of the Phthalocyanines CAS 147-14-8 on the respiration rate of activated, domestic sludge were tested by a test following ISO 8192 [BASF AG 1988]. An EC20 of ca. 750 mg/l was determined after 30 min.