Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, (1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl derivs.

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION
- Separetely for each concentration 0.50 g, 1.00 g, 50.00 g and 100.00 g test substance were added to 10 l test water and the mixture was homogenized using an ultra-turrax stirrer.
- Dispersed undissolved test substance was visible in all concentrations at the water surface increasing with the concentratinos and in the 50 and 100 mg/L group decreasing with time due to sedimentation.
- After about 24 h undissolved test substance was visible in all concentrations on the bottom increasing with the concentratinos.
- All test solutions were colored blue increasing with the concentrations.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

GENERAL INFORMATION
- Animal supplier: Bio International B.V., AP Roermond, NL
- Age of animals: about 7 months
- Body length: 2.81 (2.1 - 3.1) cm
- Body weight: 0.22 (0.14 - 0.28) g

HOUSING
- Fish were kept in a flow-through tank in tap water, not chlorinated, passed through a charcoal filter and aerated with oil-free air
- Duration of housing: about 2 months
- Photoperiod: 16 h light : 8 h dark
- Water temperature: 21 - 22 °C
- Mortality: during the last 7 days: 0 %
- Diet: "TETRA MIN" standard feed for aquarium fish, TETRA-WERKE, Melle, FRG, ad libitum; and on workdays generally additionally live brine shrimp

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22 - 23 °C

pH:

8.2 - 8.5

Dissolved oxygen:

7.5 - 9.0 mg/L

Nominal and measured concentrations:

- Concentrations tested (nominal): 50, 100, 5000 and 10000 mg/L plus control

Details on test conditions:

TEST WATER
- Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated
- Test water properties:
Total hardness: about 2.5 mmol/L
Acid capacity: about 5.5 mmol/L
Ca content: about 90 mg/L
Mg content: about 10 mg/L
pH: about 8.0 - 8.6

TEST SYSTEM
- Test vessels: all-glass aquaria (30 x 22 x 24 cm)
- Test volume: 10 L (water depth about 17 cm)
- No. animals / vessel: 10
- Loading (g fish / l water): approx. 0.2 g/L

FURTHER TESTING CONDITIONS
- Photoperiod: 16 h light : 8 h darkness
- Withdrawl of food: 1 day before and during exposure
- Aeration: slight

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

no analytical monitoring, a solubiliser was used

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

- Lot/batch No.: 7051
- Name of test material (as cited in study report): Hostaperm Blue BG

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 4 g test substance were mixed with 4 g Tween 80 and filled up with test water to 40 l
- Controls: 4 g Tween 80 mixed with 40 l reconstituted water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Source: Fisheries Research Station, University of Agricultural Sciences, Bangalore, India
- Length at study initiation (length definition, mean, range and SD): 3.1 - 4.0



ACCLIMATION
- Acclimation period: 48 h
- Type and amount of food: commercial fish feed
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): no mortality observed

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22 +/- 2 °C

pH:

7.88 +/-0.14

Dissolved oxygen:

82 +/- 8 %

Nominal and measured concentrations:

Nominal test concentrations:
control,
solvent control,
100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (40 l)
- Type (delete if not applicable): open / closed
- Fill volume: 20 l
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: max. 1 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms after 1, 2, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 100 mg/l
- Results used to determine the conditions for the definitive study: LC50 >100 mg/l

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No observation of symptoms of intoxication possible since test water was densely coloured by the test substance.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to attached justification.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Description of key information

With high probability acutely not harmful to fish.

Additional information

There are no studies available that assess the acute toxicity of the test substance to fish. However, as the test substance belongs to the substance group of Phthalocyanines whose group members resemble in their chemical structure as well as in their phys-chem properties, a read-across approach was used to cover this endpoint.

 

A study on the toxicity of CAS 59160-79-1 to fish revealed no toxicity to fish (LC50 > 10000 mg/L) at test determination after 96h. This test was run in a static system according to OECD 203 with analytical monitoring [BASF 1999].

One further  study is available for the source substance CAS 147-14-8 which was performed similar to OECD 203 using the common carp (Cyprinus carpio) as test species, too. The test concentrations were prepared using a vehicle. The 96-h LC50 was given as > 100 mg/L. Analytical monitoring was not performed.