Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 284-915-2 | CAS number: 84989-26-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 06 to March 19, 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Chromate(3-), [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)][3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)]-, sodium
- EC Number:
- 284-915-2
- EC Name:
- Chromate(3-), [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)][3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)]-, sodium
- Cas Number:
- 84989-26-4
- Molecular formula:
- C36H20CrN8Na3O14S2
- IUPAC Name:
- trisodium 12'-methyl-6',7-dinitro-14'-phenyl-2',13,15,16'-tetraoxa-1λ⁵,2,9',10'λ⁵,13',14'-hexaaza-14-chromaspiro[hexacyclo[12.11.0.0³,¹².0⁴,⁹.0¹⁶,²⁵.0¹⁹,²⁴]pentacosane-14,1'-tetracyclo[8.6.0.0³,⁸.0¹¹,¹⁵]hexadecane]-1,3,3'(8'),4',5,6',7,9,9',11,11'(15'),12',16(25),17,19,21,23-heptadecaene-1,10'-bis(ylium)-14,14,14-triuide-4',10-disulfonate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Kleintier farm Madoerin AG, CH-4414 Fuellinsdorf/Switzerland.
- Age at study initiation: 9 to 11 weeks.
- Weight at study initiation: males 201 - 245 g and female 178 - 212 g.
- Fasting period before study: fasted for 12 to 18 hours (access to water was not interrupted). Food was again presented approximately one hour after dosing.
- Housing: groups of five in Makrolon type-3 cages with standard softwood bedding. The cages were cleaned twice weekly during the test period.
- Diet: ad libitum; pelleted standard Kliba 343, Batch 36/85 and 39/86 rat maintenance diet. Food was analysed for contaminants.
- Water: ad libitum; community tap water from Itingen. Water was analysed for contaminants.
- Acclimation period: at least one week under laboratory conditions, after veterinary examination.
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 3 °C.
- Humidity: 40 - 70 %.
- Air changes: air-conditioned with 10-15 air changes per hour.
- Photoperiod: 12 hours artificial fluorescent light/12 hours dark, music/light period.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- TEST ARTICLE PREPARATION
The test article was placed into a glass beaker on a tared Mettler PK 4800 balance, and the vehicle was added.
A weight by volume dilution was prepared using a homogenizer (Ultra-Turrax, Janke and Kunkel, Staüfen, FRG).
Homogeneity of the test article in the vehicle was maintained during treatment using a magnetic stirrer.
The preparation was made immediately prior to dosing.
VEHICLE
4 % solution of CMC, carboxymethylcellulose sodium salt purum (Fluka AG, Buchs / Switzerland in distilled water). - Doses:
- Application Volume/kg body weight:
10 ml at 1000 mg/kg
20 ml at 3000 mg/kg
20 ml at 5000 mg/kg
20 ml at 8000 mg/kg - No. of animals per sex per dose:
- 5 males and 5 females per group
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations and weighing: mortality/viability were recorded four times during test day 1, and daily during days 2-15. Body weights were recorded at the test days 1 (pre-administration), at the day 8 and the day 15.
- Necropsy of survivors performed: yes. Necropsies were performed by experienced prosectors. All animals were necropsied. All animals surviving to the end of the observation period were killed by intraperitoneal injection of sodium pentobarbitone.
- Other examinations performed: each animal was examined for changes in appearance and behaviour four times during day 1, and daily during days 2-15.All abnormalities were recorded. - Statistics:
- The LOGIT-Model (COX, Analysis of Binary Data, London 1977) was applied to estimate the toxicity value. Additionally, the 90, 95 and 99 % confidence intervals for the toxicity and the slope of the concentration response line were estimated.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 5 792 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 3 813 - 14 980
- Mortality:
- 1000 mg/kg: no deaths occurred.
3000 mg/kg: one female dead at day 2 and two females dead at the day 3; no males dead.
5000 mg/kg: two females dead at day 2 and one at the day 3; no males dead.
8000 mg/kg: three females dead at day 2 and one at the day 3; two males dead at day 2 and one at the day 3. - Clinical signs:
- other: The following symptoms were observed: 1000 mg/kg: sedation, dyspnea, curved body position, ruffled fur. 3000 mg/kg: sedation, dyspnea, ataxia, curved body position, diarrhea, ruffled fur. 5000 mg/kg: sedation, dyspnea, ataxia, curved body position, diarrh
- Gross pathology:
- 1000 mg/kg killed:
lung: partly red, focal (2), partly red, maculate (1), dark-red, mottled (3), no pathologic changes (4)
3000 mg/kg dead:
lung: black, mottled (1), reddish foci/4 mm (1)
liver: black discoloured (1), black (2)
stomach/intestines : black (3)
anal region: black (1)
3000 mg/kg killed:
lung: mottled (3)
no pathologic changes (4)
5000 mg/kg dead
lung: mottled (1)
stomach/intestines : black (2), black contents (1)
5000 mg/kg killed
no pathologic changes (7)
8000 mg/kg dead
liver: black discoloured (7)
lung : mottled (5)
stomach/intestines: black (7)
8000 mg/kg killed
no pathologic changes (3)
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified as harmful or toxic according to the CLP Regulation (EC) No. 1272/2008.
- Conclusions:
- LD50: 5792 mg/kg bw (CL 95 %: 3813 - 14980 mg/kg).
- Executive summary:
Method
The test article was administered to rats of both sexes by oral gavage, at doses from 1000 to 8000 mg/kg. The study procedures followed fulfil the OECD guideline 401 and EU method B1.
Results
The following death rate was observed:
0 % at 1000 mg/kg;
30 %. at 3000 mg/kg;
30 % at 5000 mg/kg;
70 % at 8000 mg/kg.
Based on these observations, the Logit-Estimation for the acute oral toxicity in rats of both sexes observed for a period of 15 days is 5792 mg/kg with 95 % CI of males/females 3813 - 14980 mg/kg
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.