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Diss Factsheets
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EC number: 913-635-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study was non GLP, but followed ASTM guidelines. The report provided detailed information for a clear interpretation of the results.
- Justification for type of information:
- See attached document
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were collected at the median depth of the water column of each test chamber
- Vehicle:
- no
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- Bluegill sunfish were provided by the Frankfort National Fish Hatchery, Frankfort, KY. Fishes were acclimated in ASTM water for at least 2 weeks, and feeding (brine shrimp twice daily) was withheld 24 hours prior to testing.
Weight of blugill used in the acute toxicity test was 0.260 g. The standard error was 0.018g. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Mean = 101.7 mg CaCO3/L
Standard Deviation = 7.6 mg CaCO3/L - Test temperature:
- Mean = 21.7 degrees C
Standard deviation = 0.1 degrees C - pH:
- Mean = 7.58
Standard Deviation = 0.15 - Dissolved oxygen:
- Mean = 7.1 mg/L
Standard deviation = 0.3 mg/L - Nominal and measured concentrations:
- Nominal concentrations = Control, 4.8, 5.4, 6.0, 6.6, 7.2 g/L
Measured concentrations = <0.006 (Control), 4.86, 5.52, 6.09, 6.80, and 7.20 g/L - Details on test conditions:
- Sodium chloride was administered under continuous flow conditions using 8-L Pyrex exposure chambers (each containing 20 organisms). Control water and test water with specified exposure concentrations of toxicant was pumped through the exposure chambers at a rate (30 mL/min) allowing the test medium to be renewed over 5 times a day. The inlet of the exposure chamber was positioned 5 cm above the bottom of the container and the outlet, situated on the opposite side, was approximately 3 cm below the top. Peristaltic pumps (Cole-Palmer Masterflex Model 7553-10) connected to 20-L Nalgene reservoirs were used to maintain the continuous-flow system. The tests were performed in duplicate, using at exponential series of at least 5 exposure cocentrations and controls. Feeding was withheld over the 4-day exposure period.
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5 840 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 5560 - 6080 mg/L
- Details on results:
- 96 hour percent survival in the control, 4.8, 5.4, 6.0, 6.6, and 7.2 g/L treatment groups were 95, 80, 58, 45, 20, and 5%, respectively.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96 hour LC50 for bluegill sunfish (Lepomis macrochirus) exposed to NaCl in a continuous flow-through system was 5840 mg/L.
- Executive summary:
The 96 hour LC50 for bluegill sunfish (Lepomis macrochirus) exposed to NaCl in a continuous flow-through system was 5840 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Taken from OECD SIDS (Guideline study).
- Justification for type of information:
- See attached document
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- other: EPA/600/4-90/027, EPA/600/6-91/003
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- not specified
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- a) Size: Not described
b) Age: 1-7 days old
c) Pretreatment: no data
d) Supplier/Source: ENSR, Fort Collins, CO, USA - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 630 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 6 560 mg/L
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 6 660 mg/L
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
- Table: Mean LC50 values
-------------------------------------------------------
Exposure period 24h 48h 96h
Number of test 3 3 3
LC50 (mg/l)* >6660 >6560 4630
Range (mg/l)** 4700->10000 4390->10000 3930-5360
------------------------------------------------------
* Values are arithmetic mean of 3 tests.
** Range of three LC50s - Conclusions:
- The 96 hr LC50 of calcium chloride to the Fathead minnow was determined to be 4630 mg/L (expressed as anhydrous CaCl2).
- Executive summary:
The 96 hr LC50 of calcium chloride to the Fathead minnow was determined to be 4630 mg/L (expressed as anhydrous CaCl2).
Referenceopen allclose all
Description of key information
No experimental data are available that assess the short-term toxicity of the reaction mass of calcium chloride and sodium chloride to fish. Nevertheless, a 96h-LC50 value can be derived from reliable information that is available on both constituents.
Based on the data on calcium chloride, the 96h-LC50 for the reaction mass of calcium chloride and sodium chloride is considered to be 4630 mg/L (i.e. worst case).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4 630 mg/L
Additional information
No experimental data are available to assess the short-term toxicity of the reaction mass of calcium chloride and sodium chloride to fish. Nevertheless, reliable information is available for calcium chloride (CaCl2) and sodium chloride (NaCl). The acute toxicity towards fish of the constituents of the reaction mass (i.e. CaCl2 and NaCl) is known and the LD50 of the substance can thus be predicted. To this end, the key studies assessing the acute toxicity of both compounds identified in the REACH registration dossier were selected.
For calcium chloride, a short-term toxicity test with Pimephales promelas (Fathead minnow) was carried out based on EPA guidances (Mount et al. 1997). A control plus four test substance concentrations were tested (ranging from 1250 to 10000 mg/L). The experiment was run in triplicate with 5 test animals per dose group per replicate. Based on survival after 96h, the LC50 of CaCl2 for was estimated at 4630 mg/L (expressed as anhydrous CaCl2).
For sodium chloride, the LC50 for Lepomis macrochirus (Bluegill sunfish) was estimated from a non-GLP study based on ASTM guidelines (Birge et al. 1985). In this flow-through experiment, 20 fishes per dose group were exposed to five NaCl concentrations ranging from 4.8 to 7.2 g/L. The test (including control) was run in duplicate. The LC50 for bluegill sunfish was 5840 mg/L.
The reaction mass contains c.a. 68% of calcium chloride and 30% sodium chloride. The LC50 of the reaction mass was defined based on a worst case (i.e. the lowest LC50 of the two constituent was selected to reflect the LC50 of the reaction mass). Accordingly, the LC50 for fish of the reaction mass of calcium chloride and sodium chloride is 4630 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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