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EC number: 201-487-4 | CAS number: 83-56-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: only as secondary citation available: guideline study and GLP
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Naphthalene-1,5-diol
- EC Number:
- 201-487-4
- EC Name:
- Naphthalene-1,5-diol
- Cas Number:
- 83-56-7
- Molecular formula:
- C10H8O2
- IUPAC Name:
- naphthalene-1,5-diol
- Test material form:
- other: solid
- Details on test material:
- content: 99.9 %
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- no data
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- aqueous DMSO (30 %)
- Details on exposure:
- Test concentrations
were based on the acute toxicity in a pre-test, measuredat various intervals around 1 to 48 h after treatment.
In the main experiment
mice were exposed to single i.p. doses of 0, 12.5, 25 and 50 mg/kg bw. 24 h or 48 h (highest dose
only) after dosing bone marrow cells were collected. The animals of the highest dose group
were examined for acute toxic symptoms 1, 2-4, 6 and 24 h after start of treatment.
Toxicity and thus exposure of the target cells was determined by measuring the ratio
between polychromatic and total erythrocytes (PCE/TE).
Satellite groups of
3 male mice per
sampling time (20 min, 40 min, 1 h and 4 h after start of treatment) treated with 50 mg/kg
bw were included for determination of blood concentrations of 1,5-naphthalenediol.
Bone marrow preparations were stained with May-Grünwald and examined microscopically
for the PCE/TE ratio and micronuclei. 5 mice/sex/group were analysed; the remaining 6th
animals of each group were only evaluated in case a mouse died spontaneously. - Duration of treatment / exposure:
- single
- Frequency of treatment:
- once
- Post exposure period:
- as required by the guideline
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 12.5, 25.0, 50.0 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 5/sex/group
- Control animals:
- other: negative controls were in accordance with the OECD guideline
- Positive control(s):
- positive controls were in accordance with the OECD guideline
Examinations
- Tissues and cell types examined:
- bone marrow cells
- Details of tissue and slide preparation:
- Bone marrow preparations were stained with May-Grünwald and examined microscopically
for the PCE/TE ratio and micronuclei. 5 mice/sex/group were analysed;
the remaining 6th animals of each group were only evaluated in case a mouse died spontaneously. - Evaluation criteria:
- positive result: biological relevant increase in the number of micronucleated PCEs compared to concurrent vehicle controls
- Statistics:
- no data
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- not applicable
- Additional information on results:
- Treatment with 1,5-naphthalenediol did not result in substantially decreased PCE/TE ratios
compared to the untreated controls indicating that 1,5-naphthalenediol did not have
cytotoxic properties in the bone marrow.
In contrast, clinical signs like reduction in spontaneous activity, abdominal position and ruffled fur
indicating systemic toxicity were observed at all doses in most treated animals up to 24 h after start of the
treatment. 1,5-naphthalenediol could be quantified in the blood of the treated males 20 minutes after start of the
treatment but not at later time points confirming the bioavailability of 1,5-naphthalenediol.
Biological relevant increases in the number of micronucleated PCEs compared to the
concurrent vehicle controls were not found following treatment with 1,5-naphthalenediol at
any time point or dose level tested.
Conclusion
Under the experimental conditions used 1,5-naphthalenediol did not induce an increase in
bone marrow cells with micronuclei in treated mice and, consequently, is 1,5-
naphthalenediol not genotoxic (clastogenic and/or aneugenic) in bone marrow cells of mice.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the experimental conditions used 1,5-naphthalenediol did not induce an increase in
bone marrow cells with micronuclei in treated mice and, consequently, is 1,5-
naphthalenediol not genotoxic (clastogenic and/or aneugenic) in bone marrow cells of mice. - Executive summary:
According to OECD TG 474 1,5 -Naphthalenediol has been investigated for the induction of micronuclei in bone marrow cells of mice. Test concentrations were based on the acute toxicity in a pre-test, measured at various intervals around 1 to 48 h after treatment. In the main experiment mice were exposed to single i.p. doses of 0, 12.5, 25 and 50 mg/kg bw. Clinical signs like reduction in spontaneous activity, abdominal position and ruffled fur indicating systemic toxicity were observed at all doses in most treated animals up to 24 h after start of the treatment. Biological relevant increases in the number of micronucleated PCEs compared to the concurrent vehicle controls were not found following treatment with 1,5-naphthalenediol at any time point or dose level tested. therefore, under the experimental conditions used, 1,5-naphthalenediol did not induce an increase in bone marrow cells with micronuclei in treated mice and, consequently, is 1,5- naphthalenediol not genotoxic (clastogenic and/or aneugenic) in bone marrow cells of mice.
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