Reaction mass of 1-chlorobut-2-ene and 3-chlorobut-1-ene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study was performed prior to OECD guideline and GLP but follows principles thereof.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1981

Deviations:

no

GLP compliance:

no

Remarks:

prior to GLP requirements

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

EC Number:

908-820-9

Molecular formula:

not applicable

IUPAC Name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

Details on test material:

Details on Test material
- Name of test material (as cited in study report): Crotylchlorid, technisch
- Substance type: clear liquid
- Physical state: liquid
- Batch: not reported

Test animals

Species:

rat

Strain:

other: Bor: WISW (SPF TNO)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: F. Winkelmann, Borchen, Germany
- Weight at study initiation: male: 145 g, female 121 g
- Fasting period before study: 16h
- Housing: 1-5 animals per cage
- Diet (e.g. ad libitum): R10, Ssniff
- Water: libitum
- Acclimation period: 4-8 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +- 1°C
- Humidity (%): 60 +- 5%
- Air changes (per hr): 15 per hour
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

petrolatum

Details on oral exposure:

VEHICLE
details not given


MAXIMUM DOSE VOLUME APPLIED: 10 ml/kg

Doses:

251, 316, 398, 501, 631 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs up to 6h and then daily body weight day 1, 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight:

Statistics:

LD50 determination according to Litchfield and Wilcoxon, 95% confidence interval

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

385 mg/kg bw

95% CL:

324 - 488

Mortality:

males/females (out of 5)
251 mg/kg bw: 0/0
316 mg/kg bw: 1/0
398 mg/kg bw: 3/3
501 mg/kg bw: 4/5
631 mg/kg bw: 5/5
Death occurred within 24-48 hours post application

Clinical signs:

other: No macroscopic changes were seen at necropsy. Clinical symptoms included diarrhoea, ataxia, staggered gait, hunched posture, sedation. Up to ten days after treatment the animals showed signs of intoxication.

Gross pathology:

dead animals showed reddened mucosa in the stomach and gut, all other animals showed no signs.

Other findings:

- Potential target organs: CNS

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Remarks:

Migrated information

Conclusions:

Under conditions tested, the LD50 of the test substance was calculated to be 385 mg/kg body weight. Mortality is considered to be mainly due to its corrosive properties, however, clinical signs may be indicative of possible neurotoxicity.