Reaction mass of 1-chlorobut-2-ene and 3-chlorobut-1-ene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to Guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Umwelt, Raumordnung und Landwirtschaft des Landes Nordrhein-Westfalen

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

EC Number:

908-820-9

Molecular formula:

not applicable

IUPAC Name:

Reaction mass of (E)-1-chlorobut-2-ene and 3-chlorobut-1-ene

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Water solubility (under test conditions): test item reacts with water



OTHER PROPERTIES (if relevant for this endpoint)
- Adsorption characteristics:
- Toxicity to microorganisms:
- Other:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Marl-Ost municipal sewage treatment plant
- Storage conditions: aerated with moist air in the dark
- Storage length: six days with mineral medium
- Preparation of inoculum for exposure: 2x5990 ml mineral medium were inoculated with 10 ml of inoculum
- Pretreatment:
- Concentration of sludge: 4.7 mg/l suspended solids, 2.1818 g/l dry matter
- Initial cell/biomass concentration: 86 x 10^4 CFU/ml
- Water filtered: no

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 37.7 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium
- Solubilising agent (type and concentration if used): not used
- Test temperature: 21.4-22.7°C
- pH: 7.2 at the end of the test
- pH adjusted: no
- CEC (meq/100 g):
- Aeration of dilution water: no
- Suspended solids concentration: no data
- Continuous darkness: yes



TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:


SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:


CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:


STATISTICAL METHODS:

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

not performed

Test performance:

Test is considered as valid if
- the mean percentage degradation in the vessels containing the reference compound is >= 60% on the 14th day of incubation (here 90%)
- The mean amount of IC produced from the test blank controls at the end of the test is <= 15% of the organic carbon added initially as the test compound (here -2.0%)

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

20

Sampling time:

7 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

18

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

23

Sampling time:

23 d

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

25

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

71% degradation after 3 days
94% degradation after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Test substance is considered not readily biodegradable under conditions tested