Reaction mass of 2-hydroxy-1,3-propanediyl bismethacrylate and 101525-90-0

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11/1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

adopted 17. July 1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

adopted 29. December 1992

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Batch No. 790703330
Lot No. 32079289
Reactive ester content: 96.9 %
Glycerol dimethacrylate: 90.5 %
Glycerol trimethacrylate: 1.5 %
Methacrylic acid 0.2 %
4-Methoxy phenol /stabilizer): 202 ppm
Expiry date : 2000-03-09

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Activated sludge from the aeration tank of a municipal biological waste water treatment plant, not adapted, not pre-conditioned. 30 mg/1 dry matter in the final mixture

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Details on study design:

Inoculum: Activated sludge from the aeration tank of a municipal biological waste water treatment plant, not adapted, not pre-conditioned. 30 mg/1 dry matter in the final mixture ARA Werdhölzli, CH-8048 Zürich, 22 September 1999; 4.30 p.m.
Test unit: 1200 ml closed glass bottle (tightly closed with a butyl rubber stopper) containing a total volume of test solution of 400 ml
Illumination: Temperature-controlled dark room
Temperature: 22 ± 0.5 °C
Test medium: Aerobic mineral salts medium (Table 3) prepared with deionised water (conductivity: <1.5 μSlcm; DOC: <0.3 mg/1)
Feed: None, GLYCEROL-1,3-DIMETHACRYLATE and functional control as sole organic carbon sources
Test duration: 28 days
lncubation: Temperature-controlled dark room (22 ± 0.5 °C)


Test procedure
The activated sludge was used immediately after sampling from the treatment plant without adaptation.
'", Prior to the test the sludge was washed twice with tap water. To prepare the stock solution the test material was dissolved in a tenfold concentration in the test medium. An appropriate amount of this stock solution was diluted with test medium to give a final test concentration of 100 mg/l.
For each test series the following number of test flasks was set up:
T: Test suspension, containing inoculum, test medium and test substance (two replicates)
B: lnoculum blank, containing inoculum and test medium (two replicates)
R: Procedure control, containing inoculum, test medium and sodium benzoate as ready
biodegradable reference compound (one replicate)

When optional or necessary the following additional test flasks were set up

C: Abiotic sterile control: containing test substance, test medium and 0.2 mM HgCl2 as
sterilizing agent to prevent microbial decomposition

X: Toxicity control: contained inoculum + test medium + test substance + sodium
benzoate as ready biodegradable reference compound

One replicate sample of each of these controls was incubated.

The test vessels were agitated on an orbital shaker (100 r.p.m.) for a maximum test period of 28 days.
During the test the 0 2 uptake was measured by gas chromatography.
pH-value was checked at the beginning and at the end of the test and adjusted to pH 7.4 (± 0.2) with NaOH or
H2S04, if necessary.
The biodegradation of the test material was followed by 0 2 uptake measurements at frequent intervals
to allow the assessment of the 10-d window. Additionally, ultimate biodegradation was determined by
analysis of dissolved organic carbon (DOC) concentrations at the beginning and at the end of the test.

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

84

Sampling time:

28 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

72

Sampling time:

10 d

Details on results:

Significant biodegradation of the test substanee was observed after a lag phase of about 1 day.

Results with reference substance:

The positive control, sodium benzoate, reached 83 % biodegradation after 14 days, thus confirming suitability of inoculum and test conditions

02 uptake of the test units, the inoeulum blank and the corresponding degradation data.

	Inoculum blank	Test unit no 1 containing test material			Test unit no 1 containing test material
Time (days)	Total O2 uptake in test sample (mg)	Total O2 uptake in test sample (mg)	BOD (mg O2/mg substance)	Degradation (%)	Total O2 uptake in test sample (mg)	BOD (mg O2/mg substance)	Degradation (%)	Mean degradation of no. 1+2 (%)
0	0.00	0.00	0.00	0.0	0.00	0.00	0.0	0.0
1	-0.11	0.69	0.02	1.1	1.01	0.03	1.6	1.4
4	4.18	13.29	0.23	13.0	13.06	0.22	12.7	12.8
8	5.54	40.79	0.91	51.7	39.90	0.88	50.4	51.1
12	9.49	58.51	1.23	69.9	56.10	1.17	66.5	68.2
14	6.67	59.68	1.33	76.6	57.65	1.27	72.7	74.2
18	7.89	63.83	1.40	79.8	59.32	1.29	73.4	76.6
21	9.53	63.62	1.35	77.2	62.29	1.32	75.3	76.2
25	10.92	69.51	1.46	83.6	67.86	1.42	81.2	82.4
28	10.72	71.04	1.51	86.0	67.72	1.43	81.3	83.7

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

GLYCEROL-1 ,3-DIMETHACRYLATE reached the pass level of 60 %for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.

Executive summary:

The biodegradability of GLYCEROL-1,3-DIMETHACRYLATE exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure

conditions in a Manometric Respirometric Test acc. OECD 301 F. The biodegradability of GLYCEROL-1,3-DIMETHACRYLATE based on 02 consumption was calculated tobe 84 % after 28 days as compared to the theoretical 02 demand (ThOD). The biodegradation of GL YCEROL-1,3-DIMETHACRYLATE reached 72 % at the end of the 10-d window. Significant biodegradation of the test substance was observed after a lag phase of about 1 day. The procedure control sodium benzoate reached 83 % biodegradation after 14 days, thus confirming suitability of inoculum and test conditions. The calculated biodegradation based on DOC measurement reached 99 % for GLYCEROL-1,3- DIMETHACRYLATE and 99 % for sodium benzoate. The data show that the consumed 02 was used for almost complete mineralization of the test compound, since the determined degradation values based on BOD were within the same range than those based on DOC.

GLYCEROL-1,3-DIMETHACRYLATE reached the pass level of 60 % for ready biodegradability in the Manometric Respirometry Test OECD 301 F within the 10-d window and, therefore, can be termed as readily biodegradable.

Description of key information

GLYCEROL-1,3-DIMETHACRYLATE reached the pass level of 60 % for ready biodegradability in the Manometrie Respirometry Test OECD 301 F within the 10-d window and, therefore, can be termed as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable