6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:3)

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-12-13 to 1990-02-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study. Deviations from the updated OECD-guideline (adopted 2006): Percent particles < 20 µm not determined. Photoperiod 16h light/ 8h dark not maintainet from day 4 to day 14 after begin exposition.

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Deviations:

yes

Remarks:

GLP study. Deviations from the updated OECD-guideline (adopted 2006): Percent particles < 20 µm not determined. Photoperiod 16h light/ 8h dark not maintainet from day 4 to day 14 after begin exposition.

GLP compliance:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): In order to achieve the required test concentrations the dry premixes of test substance and sand were blended with the dry soil in a HOBART-blender. After dispensing this blend into the test containers the seeds were planted and covere with a thin layer of soil. The water was added subsequently
- Controls: Blank: Soil

Species:

Vicia sativa

Details on test organisms:

- Seed weight: 50.13 mg
- Seed viability: 94 %

Species:

Brassica rapa

Details on test organisms:

- Seed weight: 2.19 mg
- Seed viability: 98 %

Species:

Lolium perenne

Details on test organisms:

- Seed weight: 2.21 mg
- Seed viability: 94 %

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

18 d

Test temperature:

18 - 21°C

pH:

7.2

Details on test conditions:

TEST SYSTEM
- Testing facility: growth chamber
- No. of replicates per treatment group: 4 replicates with 5 seeds each
- No. of replicates per control: 4 replicates with 5 seeds each

SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (if artificial substrate): 80% "Loess" and 20% fine quartz sand
- Organic carbon (%): 0.23 %

GROWTH CONDITIONS
- Photoperiod: Photoperiod 16h light/ 8h dark not maintained from day 4 to day 14 after begin exposition (continous light during this period).
- Light intensity and quality: 2600 - 5000 Lux (52 - 100 µE/m2sec.)
- Air humidity (%): 56 - 83 %
- Watering regime and schedules: Daily control of weight (pot including soil, water, plant) from monday to friday and additon of water where necessary.

Nominal and measured concentrations:

1, 10, 100 mg/kg (nominal)

Species:

Lolium perenne

Duration:

4 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Brassica rapa

Duration:

3 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Vicia sativa

Duration:

4 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Species:

Lolium perenne

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Brassica rapa

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Species:

Vicia sativa

Duration:

16 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Details on results:

Emergence:
EC 0 for all three species tested: 100 mg/kg
EC 100 for all three species tested: 100 mg/kg
Growth:
EC 0 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 0 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 0 (15-16d) in the test for VICIA SATIVA: 100 mg/kg
EC 100 (15-16d) in the test for LOLIUM PERENNE: 100 mg/kg
EC 100 (15-16d) in the test for BRASSICA RAPA: 100 mg/kg
EC 100 (15-16d) in the test for VICIA SATIVA: 100 mg/kg

No visible phytotoxic effects were observed on all three species.

Description of key information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Data on the toxicity to terrestrial arthropods are only available for the amine part CAS 80584-91-4.   The test reveals a low hazard potential to terrestrial plants.

Additional information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Data on the toxicity to terrestrial arthropods are only available for the amine part CAS 80584-91-4.

The toxicity to terrestrial plants was assessed in terrestrial growth test in accordance to OECD guideline 208. Three different species Lolium perenne, Brassica rapa and Vicia sativa were exposed to artificial soil at nominal concentrations of 1, 10 and 100 mg/kg. The EC50 based on emergence and growth was determined to be > 100 mg/kg, EC0 at least 100mg/kg for all three species.