Registration Dossier
Registration Dossier
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EC number: 205-840-3 | CAS number: 155-04-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guidline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Benzothiazole-2-thiol
- EC Number:
- 205-736-8
- EC Name:
- Benzothiazole-2-thiol
- Cas Number:
- 149-30-4
- Molecular formula:
- C7H5NS2
- IUPAC Name:
- 1,3-benzothiazole-2-thiol
- Details on test material:
- 2-mercaptobenzothiazole, purity: < 96 %
Constituent 1
Method
- Target gene:
- tk locus
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- experiment I (-S9/-S9): 0, 10, 20, 30, 50, 75, 100 µg/ml; experiment II (-S9/+S9): 0, 20, 30, 40, 50, 60, 70 µg/ml
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- -S9: ethylmethanesulphonate, +S9: benzo(a)pyrene
- Positive control substance:
- other:
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: mouse lymphoma L5178Y cells
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Cytotoxicity:
experiment I
-S9: at 30 µg/ml 12 % survival
+S9: at 50 µg/ml 17 % survival
experiment II
-S9: at 70 µg/ml 20 % survival
+S9: at 60 µg/ml 17 % survival
No significant increases in mutant frequency, compared to the solvent control cultures, were observed in cultures treated with the test substance at any concentration tested in either the presence or absence of S9 -mix.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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