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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP and guidline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
GLP compliance:
yes
Type of assay:
mammalian cell gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
2-mercaptobenzothiazole, purity: < 96 %

Method

Target gene:
tk locus
Species / strain
Species / strain / cell type:
mouse lymphoma L5178Y cells
Metabolic activation:
with and without
Metabolic activation system:
S9-mix
Test concentrations with justification for top dose:
experiment I (-S9/-S9): 0, 10, 20, 30, 50, 75, 100 µg/ml; experiment II (-S9/+S9): 0, 20, 30, 40, 50, 60, 70 µg/ml
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Remarks:
-S9: ethylmethanesulphonate, +S9: benzo(a)pyrene
Positive control substance:
other:

Results and discussion

Test results
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not specified
Positive controls validity:
valid
Remarks on result:
other: strain/cell type: mouse lymphoma L5178Y cells
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Cytotoxicity:

experiment I

-S9: at 30 µg/ml 12 % survival

+S9: at 50 µg/ml 17 % survival

experiment II

-S9: at 70 µg/ml 20 % survival

+S9: at 60 µg/ml 17 % survival

No significant increases in mutant frequency, compared to the solvent control cultures, were observed in cultures treated with the test substance at any concentration tested in either the presence or absence of S9 -mix.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative