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EC number: 639-566-4
CAS number: 165184-98-5
A sediment toxicity study was performed
according to OECD Guideline 225 and in compliance with GLP. Groups of
the oligochaete, Lumbriculus variegatus, were exposed to Hexyl
Cinnamic Aldehyde in a static water-sediment system for 28 days. The
study was performed by spiking the test item into artificial sediment
with concentrations of 3.2, 10, 32, 100, 320 and 1000 mg/kg sediment dry
weight and a further equilibration period of 7 days. Six replicates (10
worms/vessel) per control, solvent control and test item concentration
were set up for biological investigations. A recovery phase of a further
28 days was also carried out by placing the groups (in duplicates) into
The concentrations of Hexyl Cinnamic
Aldehyde in the sediment and the overlying water were analytically
verified for all test item concentrations on Days 0 (worm insertion), 14
and 28 via HPLC-DAD. Additionally on Day -7 (Day of application), the
test item concentration in the spiked sediment was verified at 1000
mg/kg treatment and the control.
The measured concentration of the test item
in the spiked sediment (1000 mg/kg sediment dry weight) on day of
application (Day -7) was 92 % of the nominal value. The measured
concentrations of Hexyl Cinnamic Aldehyde in the sediment at the
beginning of the test (Day 0, after equilibration) were in the range of
26 - 81 % of the nominal values with the highest losses at low
concentrations and the lowest losses at high concentrations. At the end
of the exposure period (Day 28) the measured concentrations further
decreased to < LOQ in the lowest 2 concentrations, just 2% of the
nominal in concentrations from 32 to 320 mg/Kg dw and 13 % of the
highest concentration at 1000 mg/Kg dw. In the aqueous layer the
concentrations were constantly <LOQ during the test. This provides
further evidence that the substance would be rapidly removed in the
sediment compartment and unlikely to be transferred to the pelagic
Effects on worms were limited during the
study. No significant mortality was observed (>10%) at any
concentration. However the total number of worms was substantially lower
at 100, 320 and 1000 mg/Kg dw. The EC50 based on biomass was determined
as 659 mg/Kg dw. Nevertheless, this effect was suspected to be due to
palatability issues related to the high concentrations. At the highest
concentration of 1000 mg/Kg dw, the worms refused to burrow and in the
recovery study complete reversibility of the effects were demonstrated.
The Authors consider that the NOAEC was
therefore 1000 mg/Kg dw, however as effects were noted down to 100 mg/Kg
dw during exposure the NOEC of 32 mg/Kg is retained for this study.
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