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EC number: 639-566-4
CAS number: 165184-98-5
In an OECD 202 study on Daphnia magna performed according to GLP, effects were observed after 24 and 48 h using a flow-through method with a solvent and although floaters were observed in all trest concentrations except the control this study is considered suitable for hazard assessment .
Two OECD 202 studies on Daphnia magna were performed according to
GLP, one using a flow-through method with a solvent and one as a WSF
method with all loadings above the limit of solubility, under
semi-static conditions and in the dark. The first study using solvent
was considered valid as, further to a recent solubility study using a
slow-stir method in pure water and daphnid medium, the solubiltiy in
water was observed to be much higher than previously thought, around 1.5
mg/L. The previous lower solubility results of 0.3 mg/L were found to be
caused by loss of the test substance during centrifugation step.
In the second study which is considered a supporting study, no
significant effect (5% immobilisation) was observed after 48 h in a
"Water Soluble Fraction" (WSF) concentration of 1 mg/L which is greater
than the water solubility limit of the test substance however, centrifugation
was used prior to exposure and the substance recovery was found to be
<0.0018 mg/L (LOQ) of hexyl cinnamic aldehyde. It is likely that the
substance was lost to the centrifuge tubes (as has recently been
observed in the solubility studies) therefore this
study can only be used as a supporting study in the hazard assessment.
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