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EC number: 202-851-5 | CAS number: 100-42-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 March - 31 March 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- and: EC Guideline L383A-C.1
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- no attest of authority
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Eluate: water - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead Minnow
- Source: Springborn Laboratories, USA
- Length at study initiation (length definition, mean, range and SD): mean: 37mm, range 29 to 46 mm
- Weight at study initiation (mean and range, SD): mean 0.51 g, range 0.20 to 0.85 g
- Feeding during test
- Food type: dry commercial pellet food
- Amount: ad libitum
- Frequency: daily except during the 48-hours prior to testing
ACCLIMATION
- Health during acclimation (any mortality observed): No mortality during the 48-hour period prior to tesing - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 34 to 36 mg/L
- Test temperature:
- 21-22 °C
- pH:
- 6.9 to 7.1
- Dissolved oxygen:
- 91 to 96%
- Nominal and measured concentrations:
- Nominal: 3.9, 6.5, 11, 18, 30 and 50 mg A.I./L
Mean measured: 2.0, 4.0, 7.6, 11, 19, and 31 mg A.I./L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 3.8 L, zero headpsace
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter
- Renewal rate of test solution (frequency/flow rate): 15 x in 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 20
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.089 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 100-meter deep bedrock well
- Pesticides: less than 0.3 mg/kg
- Alkalinity: 21 to 23 mg/L
- Conductivity: 140 µmhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16h
- Light intensity: 40 to 70 footcandles
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
- Range finding study
- Test concentrations: 3.9, 6.5, 11, 18, 30, 50 mg A.I./L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: lower: 9, upper: 12 mg A.I./L
- Details on results:
- - Behavioural abnormalities: Within the concentrations 7.6, 11 and 19 mg A.I./L the fish showed loss of equilibrium and erratic swimming behaviour
- Mortality of control: 0% - Validity criteria fulfilled:
- yes
- Executive summary:
Styrole was testet for possible toxicological effects in an acute test on the Fish Pimephales promelas. The LC 50 after 96 h was found to be 10 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 96h flow -through acute fish tox test.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Eluate: water - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead Minnow
- Source: U.S. EPA Environmental Research Laboratory-Duluth and the University of Wisconsin-Superior campus
- Age at study initiation (mean and range, SD): 30d
- Length at study initiation (length definition, mean, range and SD): 19.0 mm, SD 2.404 mm
- Weight at study initiation (mean and range, SD): 0.101 g, SD 0.0398 g
- Housing: Adults were held at 25°C in flowing water with a controlled photoperiod of 16-hr light. Food: frozen adult brlne shrimp (Artemia sp.).
- Method of breeding: Culture chambers were provided with asbestos or plastic pipes (cut in half longitudinally) as spawning substrates, to which the naturally spawned and fertilized embryos became attached to the undersides. The substrates, with intact embryos, were then transferred into anothar 25°C bath where hatching occurred, or the substrates were removed just prior to hatching, then placed in a rearing bath. Larvae were fed 40- to 48-hr old brine shrimp nauplii in excess two times daily (once daily on weekends).
- Feeding during test: not fed 24 hr before or during a test - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 52.8 mg/L CaCO3
- Test temperature:
- 21.3°C
- pH:
- 7.2
- Dissolved oxygen:
- 8.1 mg/L
- Nominal and measured concentrations:
- Nominal: 3.12, 6.25, 12.5, 25 and 50 mg/L
Mean measured: 0.27, 0.73, 1.97, 6.38 and 13.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: < 0.5 g/l/day
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Diluted stocks were prepared in 18.9 L stainless steel carbonation vessels or 19 L glass bottles. Lake Superior water plus the liquid or solid toxicant were added to the vessels and stirred or blended vigorously
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Loss of equilibrium, general activity, locomotor activity, schooling behaviour, location in tank, startle response, body movements, body pigmentation, body of life fish - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.02 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Conf. lim: 3.24 to 4.99
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.32 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Remarks on result:
- other: Conf. lim: 2.75 to 4.01
- Details on results:
- - Behavioural abnormalities: Affected fish lost equilibrium prior to death
- Validity criteria fulfilled:
- yes
- Executive summary:
Styrole was testet for possible toxicological effects in an acute test on the Fish Pimephales promelas. The LC50 after 96 h was found to be 4.02 mg/L.
Referenceopen allclose all
Description of key information
Acutely toxic for fish
Styrole was testet for possible toxicological effects in an acute test on the Fish Pimephales promelas. The LC50 after 96 h was found to be 4.02 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 4.02 mg/L
Additional information
Although results on acute toxicity tests on various species of fish are available, the validity of many of these test is questionable because they were conducted in static system and without measurement of the effective substance concentration. Lack of information on potential loss of styrene due to volatilization over the course of exposure makes the interpretation of these results difficult. The flow-through studies with concentration monitoring are considered to be the most reliable results (Springborn 1995, Geiger et al. 1990). The lower of these gives an 96 -hours LC50 for fathead minnows (Pimephales promelas) of 4.02 mg/l. The study of Qureshi et al. (1982, Onchorynchus mykiss, 24 -hr LC50: 2.5 mg/l) is used as supporting the chosen value.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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