Benzene, ethylenated, residues, distn. lights

Endpoint summary

Description of key information

An acute oral toxicity study was carried out according to OECD guideline 423 and Eu method B13/14. A group  of five female and male Sprague-Dawley rats were administered a single oral dose of Ethylbenzene, manuf. of, distn. residues, distn. lights., suspended in distilled water. No death were reported during the course of the study. Clinical signs such as light purple coloured urine and brown staining to the muzzle were noted. All animals had recovered on day 4 after dosing.
Acute oral toxicity:
LD50 > 2000 mg/kg bw for rat (limit test)

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion

Dose descriptor:

LD50

Value:

2 000 mg/kg bw

Additional information

Acute Oral Toxicity Study

Pooles (2010) investigated the potential acute toxic effects of Ethylbenzene, manuf. of, distn. residues, distn. lights. to female and male Sprague-Dawley rats following exposure by oral gavage. Groups of 5 female and 5 male rats were administered a single dose of Ethylbenzene, manuf. of, distn. residues, distn. lights. in corn oil at a dose level of 2000 mg/kg bw. The rats were then observed for 14 days following test article administration. No death occurred during the course of the study.

Clinical signs were confined to confined to light purple coloured urine was reported in all animals and persisted until day 4. Salivation and brown staining to the muzzle of two males and piloerection and hunched posture observed in one male. All animals had recoved by day 4 and gained weights throughout the course of the stud.

All animals gained weight at the end of the study. No abnormalitites were recorded at necropsy. Therefore, the LD₅₀of DCI-30.n was estimated to be greater than 2000 mg/kg bw.

Justification for classification or non-classification

Based on the absence of any systemic effects in the acute oral study, the LD50 of Ethylbenzene, manuf. of, distn. residues, distn. lights. was estimated to be greater than 2000 mg/kg bw. On this basis, Ethylbenzene, manuf. of, distn. residues, distn. lights. does not warrant any classification according to both Directive 67/548/EEC and Regulation (EC) No 1272/2008.