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EC number: 253-379-1 | CAS number: 37172-53-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl 2-hexyl-3-oxocyclopentanecarboxylate
- EC Number:
- 253-379-1
- EC Name:
- Methyl 2-hexyl-3-oxocyclopentanecarboxylate
- Cas Number:
- 37172-53-5
- Molecular formula:
- C13H22O3
- IUPAC Name:
- methyl 2-hexyl-3-oxocyclopentane-1-carboxylate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Test substance storage: At room temperature in the dark
- Description: Clear colourless liquid
- Expiry date: 28 March 2009
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Phenobarbital/b-naphthoflavone induced rat liver S9 homogenate
- Test concentrations with justification for top dose:
- Dose range finding test/Experiment 1:
- TA100 and WP2uvrA: 3, 10, 33, 100, 333, 1000, 3330, and 5000 μg/plate in the absence and presence of S9-mix
- TA1535, TA1537 and TA98: 10, 33, 100, 333, 1000, and 3330 μg/plate in the absence and presence of S9-mix
Experiment 2:
- TA98 and WP2uvrA: 33, 100, 333, 1000, and 3300 μg/plate in the absence and presence of S9-mix
- TA1535, TA1537 and TA100: 33, 100, 333, 1000, and 2000 μg/plate in the absence and presence of S9-mix - Vehicle / solvent:
- Ethanol
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- methylmethanesulfonate
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: reduction of the bacterial background lawn, the increase in the size of the microcolonies and the reduction of the revertant colonies
- OTHER: In the first experiment 5% (v/v) S9-mix was used and in the second experiment 10% (v/v) S9-mix was used. - Evaluation criteria:
- Acceptability of the assay:
A Salmonella typhimurium reverse mutation assay and/or Escherichia coli reverse mutation assay is considered acceptable if it meets the following criteria: a) The negative control data (number of spontaneous revertants per plate) should be within the laboratory historical range for each tester strain. b) The positive control chemicals should produce responses in all tester strains, which are within the laboratory historical range documented for each positive control substance. Furthermore, the mean plate count should be at least three times the concurrent vehicle control group mean. c) The selected dose range should include a clearly toxic concentration or should exhibit limited solubility as demonstrated by the preliminary toxicity range-finding test or should extend to 5 mg/plate.
Data evaluation:
A test substance is considered negative (not mutagenic) in the test if: a) The total number of revertants in tester strain TA100 is not greater than two (2) times the concurrent control, and the total number of revertants in tester strains TA1535, TA1537, TA98 or WP2uvrA is not greater than three (3) times the concurrent vehicle control. b) The negative response should be reproducible in at least one independently repeated experiment. A test substance is considered positive (mutagenic) in the test if: a) The total number of revertants in tester strain TA100 is greater than two (2) times the concurrent control, or the total number of revertants in tester strains TA1535, TA1537, TA98 or WP2uvrA is greater than three (3) times the concurrent vehicle control. b) In case a repeat experiment is performed when a positive response is observed in one of the tester strains, the positive response should be reproducible in at least one independently repeated experiment. The preceding criteria were not absolute and other modifying factors might enter into the final evaluation decision.
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- DOSE RANGE FINDING TEST
- Precipitation of the test item on the plates was observed at the start of the incubation period at concentrations of 1000 µg/plate and upwards and at 3330 and 5000 µg/plate at the end of the incubation period.
- Toxicity: In tester strain WP2uvrA, no reduction of the bacterial background lawn and no biologically relevant decrease in the number of revertants were observed. In tester strain TA100, reduction of the bacterial background lawn was observed in the absence of S9-mix at concentrations of 333 µg/plate and upwards and in the presence of S9-mix at concentrations of 1000 µg/plate. Microcolonies were observed at concentrations of 3330 µg/plate and upwards.
EXPERIMENT 1
- Precipitation of the test item on the plates was observed at the start of the incubation period at concentrations of 1000 and 3330 µg/plate and at 3330 µg/plate at the end of the incubation period.
- Toxicity: In the absence of S9-mix, toxicity was observed at concentrations of 1000 µg/plate and upwards in tester strains TA1535, TA1537, and TA98. In the presence of S9-mix, toxicity was observed at concentrations of 1000 µg/plate and upwards in tester strains TA1535, TA1537 and at a concentration of 3330 µg/plate in tester strain TA98.
EXPERIMENT 2
- Precipitation of the test item on the plates was observed at the start of the incubation period at concentrations of 1000 and above and at 3330 µg/plate at the end of the incubation period.
- In tester strain WP2uvrA, no reduction of the bacterial background lawn and no biologically relevant decrease in the number of revertants were observed. In the absence of S9-mix, toxicity was observed at concentrations of 1000 µg/plate and upwards in tester strains TA1535, TA1537, TA100, and TA98. In the presence of S9-mix, toxicity was observed at concentrations of 1000 µg/plate and upwards in tester strains TA1535 and TA1537, at a concentration of 3330 µg/plate in tester strain TA98, and at a concentration 333 µg/plate and upwards in tester strain TA100.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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