4-(4-isopropoxyphenylsulfonyl)phenol

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1997-12-02 to 1998-03-19

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Type of study / information:

Endocrine system Modulation

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The study was performed to screen D-8 for estrogenic activity.
The study was conducted using a 96-well microtitre plate serial dilution procedure, based on that described by Routledge and Sumpter (1996), using a recombinant yeast, Saccharomyces cerevisiae hER, containing the human estrogen receptor gene.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(4-isopropoxyphenylsulfonyl)phenol

EC Number:

405-520-5

EC Name:

4-(4-isopropoxyphenylsulfonyl)phenol

Cas Number:

95235-30-6

Molecular formula:

C15H16SO4

IUPAC Name:

4-[4-(propan-2-yloxy)benzenesulfonyl]phenol

Details on test material:

- Name of test material (as cited in study report): D - 8
- Molecular formula: C15 H16 O4 S;
- Molecular weight: 292.4;
- Physical state: white powder;
- Analytical purity: 99.82 %;
- Purity test date: not stated;
- Lot/batch No.: 905010;
- Expiration date of the lot/batch: not stated;
- Stability under test conditions: stable at ambient temperature and conditions;
- Storage condition of test material: store in a cool dry place, protected from direct sunlight;

Results and discussion

Any other information on results incl. tables

Assessment of results:

After incubation the screen plates were read using a Titertek Multiscan MCC 340 plate reader and the colour development of the assay medium in each well measured at an absorbance of 540 nm, To correct for turbidity the screen plates were read at a second absorbance level of 620 nm.

Determination of the presence of estrogenic activity was also made by visual assessment of the screen plates and was depicted graphically by plotting corrected absorbance values at 540 nm against test concnetrations (g/L) for each test substance and reference substance.

TABLE 1

D-8 recombinant yeast screen assay – absorbance values at 540nm 

Row number	Absorbance at 540nm
	Column number
	1	2	3	4	5	6	7	8	9	10	11	12
1- Oestradiol	2.086	2.149	2.065	1.954	1.731	1.215	0.760	0.491	0.385	0.334	0.323	0.139
2- Oestradiol	1.968	1.943	1.940	1.845	1.591	1.126	0.688	0.406	0.320	0.280	0.300	0.267
3- Blank	0.345	0.254	0.250	0.251	0.243	0.262	0.246	0.224	0.237	0.241	0.304	0.258
4- Bisphenol A	1.783	1.647	1.435	1.075	0.751	0.459	0.329	0.273	0.264	0.262	0.263	0.281
5- Bisphenol A	1.808	1.658	1.375	1.077	0.744	0.450	0.336	0.286	0.267	0.255	0.285	0.286
6- Blank	0.282	0.324	0.257	0.275	0.273	0.243	0.27	0.238	0.250	0.262	0.276	0.26
7- D-8	0.240	0.323	0.255	0.273	0.260	0.286	0.274	0.278	0.268	0.253	0.298	0.306
8- D-8	0.252	0.287	0.293	0.289	0.304	0.372	0.334	0.331	0.332	0.344	0.357	0.299
Oestradiol mean	2.027	2.046	2.0025	1.8995	1.661	1.1705	0.724	0.4485	0.3525	0.307	0.3115	0.203
Bispenol A mean	1.7955	1.6525	1.405	1.076	0.7475	0.4545	0.3325	0.2795	0.2655	0.2585	0.274	0.2835
D-8 mean	0.246	0.305	0.274	0.281	0.282	0.329	0.304	0.3045	0.3	0.2985	0.3275	0.3025
Blank mean	0.3135	0.289	0.2535	0.263	0.258	0.2525	0.258	0.231	0.2435	0.2515	0.29	0.259

 

TABLE 2

 D-8 recombinant yeast screen assay –absorbance values at 620nm

Row number	Absorbance at 620nm
	Column number
	1	2	3	4	5	6	7	8	9	10	11	12
1- Oestradiol	0.366	0.460	0.399	0.405	0.401	0.335	0.311	0.301	0.286	0.257	0.245	0.123
2- Oestradiol	0.383	0.345	0.339	0.343	0.301	0.271	0.237	0.22	0.22	0.211	0.248	0.218
3- Blank	0.283	0.201	0.197	0.198	0.189	0.207	0.186	0.173	0.187	0.196	0.241	0.217
4- Bisphenol A	0.380	0.349	0.316	0.246	0.229	0.211	0.203	0.196	0.208	0.21	0.233	0.25
5- Bisphenol A	0.395	0.358	0.277	0.268	0.230	0.207	0.198	0.207	0.211	0.201	0.23	0.25
6- Blank	0.235	0.256	0.204	0.216	0.216	0.193	0.217	0.189	0.202	0.211	0.237	0.22
7- D-8	0.187	0.252	0.205	0.217	0.210	0.237	0.218	0.228	0.223	0.204	0.257	0.266
8- D-8	0.196	0.227	0.242	0.235	0.250	0.316	0.279	0.282	0.291	0.297	0.304	0.263
Oestradiol mean	0.375	0.403	0.369	0.374	0.351	0.303	0.274	0.261	0.253	0.234	0.247	0.171
Bisphenol A mean	0.388	0.354	0.297	0.257	0.230	0.209	0.201	0.202	0.210	0.206	0.232	0.250
D-8 mean	0.192	0.240	0.224	0.226	0.230	0.277	0.249	0.255	0.257	0.251	0.281	0.265
Blank mean	0.259	0.229	0.201	0.207	0.203	0.200	0.202	0.181	0.195	0.204	0.239	0.219

 

Applicant's summary and conclusion

Conclusions:

D-8 was tested for estrogenic activity in a screening test with a yeast strain containing the human estrogen receptor gene.
The results showed that D-8, tested at top concentrations of 100 mg/L did not demonstrate any evidence of estrogenic activity when screened against the human estrogen acceptor gene contained by the test organism Saccharomyces cerevisiae hER.

Executive summary:

D-8 was tested for estrogenic activity in a screening test with a yeast strain - Saccharomyces cerevisiae hER - containing the human estrogen receptor gene. Visual assessment and photometric measurement of the D-8 screen plates indicated that none of the wells containing D-8 showed any evidence of the conversion of the chromogenic substrate from yellow to red. High levels of estrogenic activity were demonstrated by the positive reference substances 17 ß-estradiol and Bisphenol A. It was concluded that D-8, tested at a top concentration of 100 mg/L, did not demonstrate any evidence of estrogenic activity when screened against the human estrogen receptor gene contained by the test organism Saccharomyces cerevisiae hER.