4-(4-isopropoxyphenylsulfonyl)phenol

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1985-03-22 to 1985-06-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Version / remarks:

May 12, 1981

Deviations:

not specified

GLP compliance:

not specified

Radiolabelling:

no

Details on sampling:

a) Test fish:
- Fish body: determined, body weight and length;
- Homogenisation (3 x) in 120 mL Acetone/120 mL Chloroform at 15000 rpm/10 min, filtration; discard residue;
- Organic layer was concentrated under reduced pressure; uptake in 80 mL n-Hexane; extraction 3x with 80 mL Acetonitrile;
separate Hexane/Acetonitrile and concentrate Acetonitrile phase under reduced pressure,
clean up by column chromatography (glass column 15 mm x 30 cm, Florisil packaging 6 g, developing solvent 1st fraction chloroform 50 mL; 2nd fraction chloroform:acetone (9:1) 50 mL);
- The test substance was eluted into the 2nd fraction, which was concentrated under reduced pressure, filled up to 30 mL with THF and subjected to HPLC analysis. Recovery rate of the test substance from the fish body was 85.1 %
- fill up by Tetrahydrofuran (30 mL);
- HPLC determination;

b) Test water:
- Sampling: 150 mL for the 1st concentration group; 750 mL for the 2nd concentration group;
- Extraction 3x with Chloroform: 100 mL for the 1st group; 150 mL for the 2nd group;
- Aqueous layer: discard;
- Chloroform layer: Concentrated under reduced pressure; filled up by tetrahydrofurane (30 mL);
- HPLC determination (20 mL for the 1st group; 10 mL for the 2nd group);
- Recovery rates from test water were 96.0 and 96.5 % for the 1st and 2nd concentration groups, respectively.

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

Dispersant and dispersing method:
Dispersant: Hardened castor oil (HCO-40);
Dispersing method: The castor oil (HCO-40, 7 g) was melted and dissolved in 20 mL of Acetone with stirring. The test substance of 1.00 g was weighed precisely, added to the acetone solution and dissolved completely. The volume of the solution was filled up to 1.000 mL with distilled water to make the stock solution of 1.000 mg/L.

Test organisms (species):

Cyprinus sp.

Details on test organisms:

TEST ORGANISM
- Common name: carp;
- Strain: not indicated;
- Source: not indicated;
- Age at study initiation: not indicated;
- Length at study initiation: average 10.5 ± 0.4 cm;
- Weight at study initiation: 23.4 ± 3.1 g;
- Weight at termination: not indicated;
- Method of breeding: not indicated;
- Health status: External disinfection by static treatment with 50 mg/L potassium permanganate for 1 h, followed by twice treatment by 50 mg/L Terramycin for 24 h,
- Description of housing/holding area: Test water tank "Flow System", made of glass; volume 100 L; Water flow volume: 300 mL/min (432 L/day);

ACCLIMATION
- Acclimation period: 20 days at 25 °C;
- Acclimation conditions (same as test or not): yes;

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Test temperature:

24.0 ± 0.8 °C

pH:

7.5 - 7.7

Dissolved oxygen:

6.6 - 7.7 mg/L

Nominal and measured concentrations:

Nominal concentrations:
1st concentration: Test substance: 0.2 mg/L (w/v); dispersant (HCO-40): 1.4 mg/L (w/v);
2nd concentration: Test substance: 0.02 mg/L (w/v); dispersant (HCO-40): 0.14 mg/L (w/v);
Actually determined:
Test groups: 1 W / 2 W / 4 W / 6 W / 8 W;
1st concentration: 0.17 / 0.18 / 0.18 / 0.18 / 0.17 mg/L (w/v);
2nd concentration: 0.016 / 0.016 / 0.017 / 0.018 / 0.017 mg/L (w/v);

Lipid content:

4.2 %

Time point:

start of exposure

Remarks on result:

other: Lipid content was determined by chloroform-methanol extraction (CBC) method.

Key result

Type:

BCF

Value:

> 27 - < 78

Basis:

whole body w.w.

Time of plateau:

28 d

Calculation basis:

steady state

Remarks on result:

other: Conc. in environment / dose: 0.2 mg/L

Key result

Type:

BCF

Value:

> 40 - < 132

Basis:

whole body w.w.

Time of plateau:

42 d

Calculation basis:

steady state

Remarks on result:

other: Conc. in environment / dose: 0.02 mg/L

Test Results

 

Elapsed days	1st Concentration group: Bioaccumulation factor		2nd Concentration group: Bioaccumulation factor
	A	B	A’	B’
3	27	35	40	51
7	29	61	40	63
14	36	70	62	91
21	58	78	90	86
28	57	50	86	109
42	39	36	132	78
56	40	41	84	129

 

(Note) The marks A, B, A’, B’ mean the ID numbers of the test fish, given at the sampling times.

Recovery of the test item from the test water was between 94.4 and 97.7 %. A recovery test from the fish body was performed with 4 fish. The recovery was determined to be between 80.5 and 88.9 %.

Validity criteria fulfilled:

not specified

Conclusions:

The BCF for D-8 at a concentration level of 0.2 mg/L were determined to be 27 - 78 at 28 days and at concentration level of 0.02 mg/L to be 40 - 132 at 42 days, respectively.

Executive summary:

D-8 was tested for bioaccumulation in fish in a method similar to OECD Guideline 305C: " Bioaccumulation: Degree of Bioconcentration in Fish", May 1981. D-8 was tested in the concentrations of 0.2 mg test item/L and 0.02 mg test item/L in a continuous flow-through system. The determination of the test item was performed by HPLC analysis. In the first concentration group of 0.2 mg test item/L, bioconcentration factors BCFs of 27 to 78 with a plateau time of 28 days were detected. In the second concentration group of 0.02 mg test item/L, BCFs of 40 to 132 with a plateau time of 42 days could be detected.

Description of key information

The BCF for D-8 at a concentration level of 0.2 mg/L were determined to be 27 - 78 at 28 days and at concentration level of 0.02 mg/L to be 40 - 132 at 42 days, respectively. The BCF of 132 was chosen as a keay value for further risk assessment. This values is seen as the worst case.

Key value for chemical safety assessment

BCF (aquatic species):

132 dimensionless

Additional information

D-8 was tested for bioaccumulation in fish in a method similar to OECD Guideline 305C: " Bioaccumulation: Degree of Bioconcentration in Fish", May 1981. D-8 was tested in the concentrations of 0.2 mg test item/L and 0.02 mg test item/L in a continuous flow-through system. The determination of the test item was performed by HPLC analysis. In the first concentration group of 0.2 mg test item/L, bioconcentration factors BCFs of 27 to 78 with a plateau time of 28 days were detected. In the second concentration group of 0.02 mg test item/L, BCFs of 40 to 132 with a plateau time of 42 days could be detected.