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EC number: 946-959-7
CAS number: -
The test substance, Acid Black 227, was tested in acute immobilisation test on Daphnia magna.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test substance nominal concentrations 1 – 100 mg·L-1. The test was performed as static. Based on no toxicity of the test substance found in the preliminary test, the study was terminated. The experiment with the concentration of 100 mg·L-1 included in the preliminary test has been performed using 20 daphnia, there through the conditions of the limit test given in guideline have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.
The samples for analytical determination were taken at the beginning and at the end of the test. The analytical results from the preliminary test showed, that the test substance, Acid Black 227, was stable in the dilution water at conditions of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values. The nominal concentrations were used for all evaluation and results.
24 hour – EC50 > 100 mg·L-1 (nominal concentration)
48 hour – EC50 > 100 mg·L-1 (nominal concentration)
24 hour – EC0 = 100 mg·L-1 (nominal concentration)
48 hour –EC0 = 100 mg·L-1 (nominal concentration)
24 hour – EC100 > 100 mg·L-1 (nominal concentration)
48 hour – EC100 > 100 mg·L-1 (nominal concentration)
The test substance was tested in acute immobilisation test on Daphnia magna according to method C.2 – Daphnia sp. Acute Immobilisation Test (GLP study). The test was performed as static. The nominal concentration were used for all evaluation and results.
24 hour–EC50>100 mg·L-1 (nominalconcentration)
48 hour–EC50>100 mg·L-1 (nominalconcentration)
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