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EC number: 947-957-9
CAS number: -
Short-term toxicity to fish:
Oncorhynchus mykiss; OECD TG 203; read-across.
96-h LL50 value of greater than 100 mg/L loading rate WAF
Short-term toxicity to aquatic invertebrates:
Daphnia magna; OECD TG 202.
48-h EC50 > 5.62 mg/L (measured) (100 mg/L nominal loading rate WAF)
48-h NOEC = 5.62 mg/L (measured) (100 mg/L nominal loading rate WAF)
Toxicity to aquatic algae:
Pseudokirchneriella subcapitata; OECD TG 201
Results based on initial nominal loading rates (WAFs):
72 -h ErL50 > 100 mg/L; 72 -h EγL50 = 37.8 mg/L; 72 -h EbL50 = 36.51 mg/L
72 -h NOErLR = 12.5 mg/L; 72 -h NOEγLR = 6.25 mg/L; 72 -h NOEbLR = 6.25
Results based on initial measured concentrations:
72 -h ErC50 > 28.92 mg/L; 72 -h EγC50 = 15.70 mg/L; 72 -h EbC50 = 15.29
72 -h NOErC = 6.53 mg/L; 72 -h NOEγC = 3.71 mg/L; 72 -h NOEbC = 3.71 mg/L
No study is available for Phosphoric acid, C8-12 (even numbered) linear
alkyl esters. Results are read across from an OECD TG
203 study performed on the 2-ethylhexyl- analogue. In this study, groups
of 7 rainbow trout (Oncorhynchus mykiss) were exposed, in groups of
seven, to a Water Accommodated Fraction (WAF) of the test item, at a
single nominal loading rate of 100 mg/l for a period of 96 hours under
semi-static test conditions. The number of mortalities and any
sub-lethal effects of exposure in each test and control vessel were
determined 3 and 6 hours after the start of exposure and then daily
throughout the test until termination after 96 hours. The 96-h LL50
based on nominal loading rates was > 100 mg/l loading rate WAF and
correspondingly the No Observed Effect Loading rate was 100 mg/l loading
Acute toxicity of Phosphoric acid, C8-12 (even numbered) linear alkyl
esters on Daphnia magna was assessed in an Acute immobilisation test,
over an exposure period of 48 hours in a static test system (OECD TG
202). Because no toxic response was observed during the preliminary
range-finding test, a Limit Test was carried out using only one test
concentration at the solubility level of the test item (100 mg/L nominal
loading rate WAF) and one control group in the definitive test.
The test concentration was analytically determined at the start and at
the end of the experiment. Measured concentration was 4.83 mg/L at the
start and 6.55 mg/L at the end of the experiment, thus the corresponding
measured geometric mean test item concentration was 5.62 mg/L. The
biological results are based on the measured geometric mean test item
Twenty animals, divided into four groups (glass beaker) of five animals
each were used at the test concentration and for the control.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study
the observed endpoints for the effect of Phosphoric acid, C8-12 (even
numbered) alkyl esters were the followings:
The 24h and 48h EC50 value: > 5.62 mg/L (measured)
The 48h EC100 value: > 5.62 mg/L (measured)
The 48h No-Observed Effect Concentration (NOEC): 5.62 mg/L (measured)
The 48h Lowest Observed Effect Concentration (LOEC): > 5.62 mg/L
The effect of the test item was assessed on algal growth using the
unicellular green alga Pseudokirchneriella subcapitata (Selenastrum
capricornutum), over an exposure period of 72 hours (OECD TG 201).
A significant toxic response was observed during the preliminary
range-finding test, therefore five test concentrations in a geometric
series with a separation factor of 2.0 and one untreated control were
tested in the main experiment.
The concentrations of test item used in the main experiment were: 6.25,
12.5, 25.0, 50.0 and 100.0 mg/L nominal loading rates WAFs. Test
concentrations were analytically determined at the start of the test and
at 24 hour intervals thereafter in order to better define loss of the
test substance during the exposure period.
The test design included three replicates at each test concentration and
six replicates for the untreated controls. There was an additional
replicate without algae at each test concentration level for further
analytical measurements (0, 24, 48 and 72 hours) in order to distinguish
degradation and adsorption of the test item to algae.
According to the relevant guideline (OECD 201) disappearance of the test
substance from solution by absorption to the increasing algal biomass
does not mean that it is lost from the test system. When the result of
the test was analysed, it was checked whether a decrease in
concentration of the test substance in the course of the test was
accompanied by a decrease in growth inhibition.
The corresponding measured geometric mean test item concentrations were:
1.78; 2.02; 8.95; 18.33 and 25.38 mg/L in presence of algae (more
details are given in 4.2.). In addition, results of the analytical
measurements of samples in absence of algae showed measured geometric
mean test item concentrations of 3.46; 6.15; 11.99; 18.18 and 24.79 mg/L
for the nominal concentrations of 6.25, 12.5, 25.0, 50.0 and 100.0 mg/L
nominal loading rate WAFs respectively (more details are given in 4.2.).
However based on the section-by-section average specific growth rates,
it is indicating that a decrease of concentration of the test item was
not clearly accompanied by a decrease in growth inhibition. Based on
these results most probably the test item is indeed absorbed to the
increasing algal biomass, and should not be considered as lost from the
Therefore it is recommended to express the EL50/EC50 and
NOELR/NOEC//LOELR/LOEC values in the initial test item concentrations
(nominal or measured) and not in the mean measured concentrations from
the algae containing samples, thus biological results are related to the
initial nominal loading rates (WAFs) and initial measured test item
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