Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test substance was added directly to the test media a) without additional ultracentrifugation at 10000 mg/L product; b) with additional ultracentrifugation (15 min) at 3000 mg/L product.

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Species: Pseudomonas putida, Strain Berlin 33/2 (DSM 50026)
Origin: Stammsammling TFB-Mirkobiologie, Henkel KGaA

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Hardness:
not specified
Test temperature:
room temperature (not specified)
pH:
not specified
Dissolved oxygen:
> 2 mg/L
Nominal and measured concentrations:
Nominal: 0, 3000, 10000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass vials
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml fill volume
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to DIN 38412 - part 27

OTHER TEST CONDITIONS
- Adjustment of pH: not indicated
- Photoperiod: not relevant
- Light intensity: not indicated


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption at 3 h
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 3 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Reported statistics and error estimates:
The calculation of the oxygen consumption and the mean values was carried out using a LOTUS calculation program.

Applicant's summary and conclusion