Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Source of Inoculum: activated sludge from a STP effluent from the STP Hochdahl (1 ml/L culture solution), with primarliy munucipal sewage effluent.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
2 - 5 mg/L
Based on:
act. ingr.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: according to OECD 301 D
- Additional substrate:
- Solubilising agent (type and concentration if used): Disponil NP 9.5EO 5PO
- Test temperature: troom temperature

- pH: not indicated
- pH adjusted: not indicated
- Suspended solids concentration:


TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 2 per concentration
- Method used to create aerobic conditions: stirring
- Test performed in open system: no


SAMPLING
- Sampling frequency: after 0, 7, 21, 28 d
- sampling storage before analysis: no

CONTROL AND BLANK SYSTEM
- Inoculum blank: 4
- Solvent control: 2 per concentration
- Reference substance: 2

STATISTICAL METHODS: not applicable
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
< 0
Sampling time:
0 d
Remarks on result:
other:
Remarks:
Based on test 2 mg/L AS
Key result
Parameter:
% degradation (O2 consumption)
Value:
70 - 80
Sampling time:
7 d
Remarks on result:
other:
Remarks:
Based on test 2 mg/L AS
Parameter:
% degradation (O2 consumption)
Value:
80 - 90
Sampling time:
21 d
Remarks on result:
other:
Remarks:
Based on test 2 mg/L AS
Parameter:
% degradation (O2 consumption)
Value:
90 - 100
Sampling time:
28 d
Remarks on result:
other:
Remarks:
Based on test 2 mg/L AS
Details on results:
degradation profile of 5 mg/L AS:
day 7: 60 - 70 %
Day 21: 60 - 70 %
day 28: 60 - 70 %

BOD5 / COD results

Results with reference substance:
sodium benzoate:
day 7: 70 - 80 %
day 21: 90 - 100 %
day 28: 90 - 100 %

Applicant's summary and conclusion