Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
test duration was only 48 h instead of 96 h
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Preparation of the test solution:
Direct weight of the test substance into the test vessels, filled up to final volumes of 10 l with copper- and chlorine-free drinking water, then stairing 5 mitmtes by ultraturrax.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden Orfe
- Source: not indicated
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): about 6 cm


ACCLIMATION
- Acclimation period: according to OECD 203
- Acclimation conditions (same as test or not):according to OECD 203
- Type and amount of food during acclimation: according to OECD 203
- Feeding frequency during acclimation:according to OECD 203
- Health during acclimation (any mortality observed): not indicated


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
about 16 °dH ( about 93 mg Ca and 12 mg Mg per litre)
pH:
7.6.-8.4
Dissolved oxygen:
76 - 94 % O2 saturation
Nominal and measured concentrations:
Nominal: 1000, 3000, 10000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L basins
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 L
- Aeration: not indicated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 203
- Intervals of water quality measurement: not indicated

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16: 8 h day night regime
- Light intensity: not indicated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms of intoxication

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx 3

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Additional remarks: The fish did not show any abnormal behaviour.
Reported statistics and error estimates:
Not applicable, due to the absence of any toxic effect uo to the highest concentration tested.

Applicant's summary and conclusion