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EC number: 226-430-0 | CAS number: 5398-29-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- adopted in 1979
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 1.56, 25 and 100 mg/L treatments
- Sampling method: Samples were taken at the beginning and at the end of the test. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding 125 mg test substance to 1L of demineralized water and stirring for about 10 minutes at 22 °C. The stock solution was subsequently diluted with demineralized water to prepare the test concentrations. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- -Test species and strain: Desmodesmus subspicatus CHODAT (SAG 86.81)
- Source (laboratory, culture collection): obtained at regular intervals from SAG and kept in liquid culture in the in-house lab - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 2 °C
- pH:
- 7.8 - 9.3
- Nominal and measured concentrations:
- Nominal: 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
Measured concentrations: The concentration control analysis were performed with test solutions of the following nominal concentrations of the test substance: 0 (control), 1.56, 25, 100 and 125 mg/L. The measured concentrations were determined to be: < 1, 1.6, 26.3, 106.8 and 116.6 mg/L (mean values) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks plugged with gas permeable siliconesponge caps containing 100 mL test solution
- Initial cells density: 10000 cells/mL
- Control end cells density: 57-fold
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH values were measured at the beginning and at the end of the test.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: permanent illumination
- Light intensity and quality: 120 µE/m2*s (400-700 nm)
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: after 72h in a counting chamber (Neubauer improved)
- Chlorophyll measurement: In vivo Chlorophyll-a fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) was measured using an EOS Filterfluorometer after 0, 24, 48 and 72h exposure time. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.77 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Any stimulation of growth found in any treatment: no - Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance did not show to be acutely harmful (72h-EC50 > 100 mg/L) but harmful at chronic exposure to freshwater algae (72h-EC10 = 14 mg/L) in a GLP guideline study using Desmodesmus subspicatus as test organism.
Reference
Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
Description of key information
EC50 (72h) > 100 mg/L, EC10 (72h) = 14 mg/L (nominal, analytically verified) for Desmodesmus subspuicatus (EU method C.3)
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 14 mg/L
Additional information
One study investigating a toxicity of 3-(amidinothio)propionic acid (CAS 5398-29-8) to freshwater algae is available. The study was performed according to GLP and EU method C.3 using Desmodesmus subspicatus as test organism (BASF 1997). Test concentrations between 0.39 and 100 mg/L were tested. Nominal concentrations were verified analytically. The 72h-EC50 value is determined to be > 100 mg/L and the 72h-EC10 value is determined to be 14 mg/L based on nominal concentrations.
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