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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 1,56 and 100 mg/L treatment
- Sampling method: samples were taken at the beginning and at the end of the test period.
- Sample preparation: Standards were dissolved and diluted in the carrier after addition of a solution of internal standard the samples were injected directly.
- Storage condition of samples: Refrigerator
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding 100 mg/L of test substance to water and stirring for 20 min at 20 °C. The test concentrations were prepared by subsequent dilution of the stock solution.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.20 - 3.20 mmol/L
Test temperature:
20 ± 2 °C
pH:
7.9 - 8.5
Dissolved oxygen:
9.2 - 15.3 mg/L
Nominal and measured concentrations:
Nominal: 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
Measured corresponding to following nominal concentrations: 0 (control), 1.56 and 100 mg/L: < 1, 2 and 92 mg/L (mean value)
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 mL test tubes with 10 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium according to guideline
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature was recorded after 0, 24 and 48h test duration. Oxygen and pH were measured at the beginning and at the end of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: 5-6 µE/m2*s (400-700 nm)

EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 3, 6, 24 and 48h exposure time.

Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
20.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
For the statistical evaluation of the data and calculation of the EC50 the Moving Average method was used.

Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.

Validity criteria fulfilled:
yes
Conclusions:
The test substance showed to be acutely harmful to aquatic invertebrates (48h-EC50 = 20.8 mg/L) in a GLP guideline study using Daphnia magna as test organism.

Description of key information

EC50 (48h) = 20.8 mg/L (nominal, analytically verified) for Daphnia magna (EU method C.2)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
20.8 mg/L

Additional information

One study investigating the short-term toxicity of 3-(amidinothio)propionic acid (CAS 5398-29-8) to aquatic invertebrates is available. The study was performed according to GLP and EU method C.2 using Daphnia magna as test organism (BASF 1995). Test concentrations between 1.56 and 100 mg/L were tested. Nominal concentrations were verified analytically. The 48h-EC50 value is determined to be 20.8 mg/L based on nominal concentrations.